SCN4B Recombinant antibody, PBS Only

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  WB analysis using 83027-4-RR (same clone as 83027-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83027-4-RR (SCN4B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83027-4-PBS in a different storage buffer formulation.

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  IHC staining of rat dorsal root ganglion using 83027-4-RR (same clone as 83027-4-PBS)

  Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglion tissue slide using 83027-4-RR (SCN4B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83027-4-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83027-4-RR (same clone as 83027-4-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human SCN4B (83027-4-RR, Clone:230297B11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83027-4-PBS in a different storage buffer formulation.

SCN4B Uni-rAb^TM Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA

Cat No. 83027-4-PBS

Clone No.230297B11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

Sodium channel subunit beta-4, Sodium channel subunit beta 4, 230297B11

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Product Information

83027-4-PBS targets SCN4B in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: SCN4B fusion protein Ag34123
• Full Name: sodium channel, voltage-gated, type IV, beta
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: NM_174934.3
• Gene Symbol: SCN4B
• Gene ID (NCBI): 6330
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8IWT1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Mammalian sodium channels are heterotrimers, composed of a central, pore-forming alpha subunit and two auxiliary beta subunits (PMID: 11486343). SCN4B (sodium channel subunit beta-4) is one of several sodium channel beta subunits, which modulate channel gating kinetics. SCN4B has an N-terminal signal peptide, a large extracellular domain predicted to form an Ig-like fold, a single transmembrane alpha helix, and a short cytoplasmic C terminus. Defects in SCN4B gene are a cause of long QT syndrome type 10 (LQT10).