Nav1.5 Polyclonal antibody

Nav1.5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

13

Reviews (2)

Cat no : 23016-1-AP

Synonyms

CDCD2, CMD1E, CMPD2, HB1, HB2, HBBD, HH1, ICCD, IVF, LQT3, Nav1.5, PFHB1, SCN5A, SSS1

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Validation Data Gallery

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  WB analysis of mouse heart using 23016-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 23016-1-AP (Nav1.5 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse skeletal muscle using 23016-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 23016-1-AP (Nav1.5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 23016-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 23016-1-AP (Nav1.5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse heart tissue
• Positive IHC detected in: mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

The immunogen of 23016-1-AP is Nav1.5 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Nav1.5 fusion protein Ag19275
• Full Name: sodium channel, voltage-gated, type V, alpha subunit
• Calculated Molecular Weight: 2016 aa, 227 kDa
• Observed Molecular Weight: 227 kDa
• GenBank Accession Number: BC140813
• Gene Symbol: SCN5A
• Gene ID (NCBI): 6331
• RRID: AB_2879198
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Voltage-gated sodium channels are responsible for initiation and propagation of action potentials in the membranes of neurons and most electrically excitable cells (PMID: 10798388). These channels are composed of a large alpha subunit that forms the ion conduction pore and auxiliary beta subunits (PMID: 11486343). The alpha subunits form a gene family with at least 10 members. Nav1.5, encoded by the SCN5A gene in humans, is a pore forming alpha subunit of voltage-gated sodium channels. Nav1.5 is the principal Na+ channel isoform expressed in cardiomyocytes. Mutations in SCN5A gene have been linked to many cardiac electrical disorders, including the congenital and acquired long QT syndrome, Brugada syndrome, conduction slowing, sick sinus syndrome, atrial fibrillation, and dilated cardiomyopathy (PMID: 23123192).

Publications

Species	Application	Title
		Cancer Lett; Sialyltransferase ST3GAL4 confers osimertinib resistance and offers strategies to overcome resistance in non-small cell lung cancer; Authors - Rui Han
human		Front Pharmacol; Spider venom-derived peptide JZTX-14 prevents migration and invasion of breast cancer cells via inhibition of sodium channels; Authors - Wenfang Wu
rat	WB	Hum Mol Genet; Mice lacking MBNL1 and MBNL2 exhibit sudden cardiac death and molecular signatures recapitulating myotonic dystrophy.; Authors - Kuang-Yung Lee
rat	WB,IF	Eur J Pharmacol; Glucose fluctuations aggravated the late sodium current induced ventricular arrhythmias via the activation of ROS/CaMKII pathway; Authors - Feng Li
mouse	WB	Biochim Biophys Acta Mol Basis Dis; Changes in ion channel expression and function associated with cardiac arrhythmogenic remodeling by Sorbs2.; Authors - Ling-Ling Qian
human	WB	J Biol Chem; Mechanistic insights into the interaction of the MOG1 protein with the cardiac sodium channel Nav1.5 clarify the molecular basis of Brugada syndrome.; Authors - Gang Yu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Udesh (Verified Customer) (01-19-2023)

Used at 1:500 dilution in 5% BSA and probed overnight at 4 degrees C. Upper membrane piece shows Nav1.5 bands close to 150 kD. Multiple bands seen probably due to BSA.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500

Nav1.5 Antibody Western Blot validation (1:500 dilution) in (Cat no:23016-1-AP)

FH

Rebecca (Verified Customer) (03-16-2022)

10ug of protein was loaded. Transference at 4ºC and 90V, for 2h. Antibody incubation ON at 4ºC.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: HEK-293T

Nav1.5 Antibody Western Blot validation (1:5000 dilution) in HEK-293T (Cat no:23016-1-AP)