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Nav1.5 Antibody 2 Publications

Rabbit Polyclonal| Catalog number: 23016-1-AP

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Con: 90 μg/150 μl

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Tested Applications

Applications:
WB, IHC, ELISA
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Images See all

Nav1.5 Antibody WB mouse heart tissue 23016-1-AP

mouse heart tissue were subjected to SDS PAGE followed by western blot with 23016-1-AP( Nav1.5 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours

Nav1.5 Antibody IHC mouse skeletal muscle tissue 23016-1-AP

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 23016-1-AP (Nav1.5 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Nav1.5 Antibody IHC mouse skeletal muscle tissue 23016-1-AP

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 23016-1-AP (Nav1.5 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, mouse
Positive WB detected in:
mouse heart tissue
Positive IHC detected in:
mouse skeletal muscle tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:50-1:500
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Nav1.5 fusion protein Ag19275
Full name:
sodium channel, voltage-gated, type V, alpha subunit
Calculated molecular weight:
2016aa,227 kDa
Observed molecular weight:
227 kDa
GenBank accession number:
BC140813
Gene ID (NCBI):
6331
Gene symbol
SCN5A
Synonyms
CDCD2, CMD1E, CMPD2, HB1, HB2, HBBD, HH1, ICCD, IVF, LQT3, Nav1.5, PFHB1, SCN5A, SSS1

Background

Voltage-gated sodium channels are responsible for initiation and propagation of action potentials in the membranes of neurons and most electrically excitable cells (PMID: 10798388). These channels are composed of a large alpha subunit that forms the ion conduction pore and auxiliary beta subunits (PMID: 11486343). The alpha subunits form a gene family with at least 10 members. Nav1.5, encoded by the SCN5A gene in humans, is a pore forming alpha subunit of voltage-gated sodium channels. Nav1.5 is the principal Na+ channel isoform expressed in cardiomyocytes. Mutations in SCN5A gene have been linked to many cardiac electrical disorders, including the congenital and acquired long QT syndrome, Brugada syndrome, conduction slowing, sick sinus syndrome, atrial fibrillation, and dilated cardiomyopathy (PMID: 23123192).


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