|Positive WB detected in||mouse lung tissue|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2400|
|Immunohistochemistry (IHC)||IHC : 1:50-1:200|
|Sample-dependent, check data in validation data gallery|
13943-1-AP targets SCNN1G in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SCNN1G fusion protein Ag5020|
|Full Name||sodium channel, nonvoltage-gated 1, gamma|
|Calculated molecular weight||74 kDa|
|Observed molecular weight||70-85 kDa|
|GenBank accession number||BC059391|
|Gene ID (NCBI)||6340|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
SCNN1G (sodium channel, nonvoltage-gated 1, gamma), also known as ENaC gamma (epithelial Na(+) channel subunit gamma) or amiloride-sensitive sodium channel subunit gamma, is the gamma subunit of the epithelial Na(+) channel (ENaC). ENaC is expressed in the apical membrane of salt-absorbing epithelia of kidney, distal colon, and lung. ENaC is a non-voltage gated, constitutively active channel highly selective for sodium. It has an essential role in salt and fluid homeostasis across epithelial tissues. Mutations in the gene of SCNN1G have been associated with Liddle syndrome. Native SCNN1G has a calculated molecular weight of 74 kDa and maybe undergo post-transcriptional modifications, including glycosylation and proteolytic cleavage (PMID: 12871941; 18086683).
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