SCNN1G Polyclonal antibody

SCNN1G Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 13943-1-AP

Synonyms

ENaCg, ENaCgamma, Gamma ENaC, Gamma NaCH, PHA1, SCNEG, SCNN1G



Tested Applications

Positive WB detected inmouse lung tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:50-1:200
Sample-dependent, check data in validation data gallery

Product Information

13943-1-AP targets SCNN1G in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SCNN1G fusion protein Ag5020
Full Name sodium channel, nonvoltage-gated 1, gamma
Calculated molecular weight 74 kDa
Observed molecular weight 70-85 kDa
GenBank accession numberBC059391
Gene symbol SCNN1G
Gene ID (NCBI) 6340
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SCNN1G (sodium channel, nonvoltage-gated 1, gamma), also known as ENaC gamma (epithelial Na(+) channel subunit gamma) or amiloride-sensitive sodium channel subunit gamma, is the gamma subunit of the epithelial Na(+) channel (ENaC). ENaC is expressed in the apical membrane of salt-absorbing epithelia of kidney, distal colon, and lung. ENaC is a non-voltage gated, constitutively active channel highly selective for sodium. It has an essential role in salt and fluid homeostasis across epithelial tissues. Mutations in the gene of SCNN1G have been associated with Liddle syndrome. Native SCNN1G has a calculated molecular weight of 74 kDa and maybe undergo post-transcriptional modifications, including glycosylation and proteolytic cleavage (PMID: 12871941; 18086683).

Protocols

Product Specific Protocols
WB protocol for SCNN1G antibody 13943-1-APDownload protocol
IHC protocol for SCNN1G antibody 13943-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Int J Mol Sci

MST3 Involvement in Na+ and K+ Homeostasis with Increasing Dietary Potassium Intake.

Authors - Chee-Hong Chan
mouseWB

Exp Anim

Oxymatrine attenuates lipopolysaccharide-induced acute lung injury by activating the epithelial sodium channel and suppressing the JNK signaling pathway.

Authors - Bingji Jin
mouseWB,IHC

Respir Physiol Neurobiol

Contribution of concentration-sensitive sodium channels to the absorption of alveolar fluid in mice.

Authors - Teruki Hagiwara
humanIHC

PLoS One

Reduced Expression of Enac in Placenta Tissues of Patients with Severe Preeclampsia Is Related to Compromised Trophoblastic Cell Migration and Invasion during Pregnancy.

Authors - Wang Shan S
mouseWB

Hypertension

Renal Natriuretic Peptide Receptor-C Deficiency Attenuates NaCl Cotransporter Activity in Angiotensin II-Induced Hypertension.

Authors - Shuai Shao