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OXCT1 Antibody 16 Publications

Rabbit Polyclonal| Catalog number: 12175-1-AP

Featured Product KD/KO validated

1 customer review

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Con: 38 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

OXCT1 Antibody WB mouse brain tissue 12175-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 12175-1-AP (OXCT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours

OXCT1 Antibody IF MCF-7 cells 12175-1-AP

Immunofluorescent analysis of MCF-7 cells, using OXCT1 antibody 12175-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

OXCT1 Antibody IP mouse brain tissue 12175-1-AP

IP Result of anti-OXCT1 (IP:12175-1-AP, 3ug; Detection:12175-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

OXCT1 Antibody IHC human gliomas tissue 12175-1-AP

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12175-1-AP( OXCT1 antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

OXCT1 Antibody IHC human gliomas tissue 12175-1-AP

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12175-1-AP( OXCT1 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse brain tissue
Positive IP detected in:
mouse brain tissue
Positive IHC detected in:
human gliomas tissue, mouse heart tissue, mouse brain tissue, human prostate cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
MCF-7 cells
Recommended dilution:
WB : 1:1000-1:8000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500
IF : 1:10-1:100
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
OXCT1 fusion protein Ag2818
Full name:
3-oxoacid CoA transferase 1
Calculated molecular weight:
520aa,56 kDa
Observed molecular weight:
56 kDa
GenBank accession number:
BC009001
Gene ID (NCBI):
5019
Gene symbol
OXCT1
Synonyms
3 oxoacid CoA transferase 1, OXCT, OXCT1, SCOT, SCOT s

Background

3-oxoacid-CoA transferase 1 (OXCT1), encoded by nuclear gene, is a mitochondrial CoA transferase required for ketone body degradation. It catalyzes the transfer of CoA from succinyl-CoA to acetoacetate, generating acetoacetyl-CoA. OXCT1 is expressed in brain, heart, and skeletal muscle, but not in liver. This antibody specifically recognizes endogenous OXCT1. (21209089)

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

OXCT1 Rabbit Polyclonal antibody

Kerry (Verified Customer), 09-26-2019
Applications
Immunohistochemistry,
No image submitted with review

No image submitted with review

Cell/Tissue Type
mouse
Lot Number
00040905
Too much background. We tried all the dilutions you recommended in your literature and then more outside of both ends of that range.
Proteintech Response
We are sorry to hear that you had problems with this product. We will run this antibody through our QC process again and will be in contact about receiving a refund.

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