Tested Applications
| Positive WB detected in | mouse brain tissue, HeLa cells, mouse skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human gliomas tissue, mouse heart tissue, mouse brain tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 10 publications below |
| WB | See 48 publications below |
| IHC | See 3 publications below |
| IF | See 8 publications below |
Product Information
12175-1-AP targets OXCT1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | OXCT1 fusion protein Ag2818 Predict reactive species |
| Full Name | 3-oxoacid CoA transferase 1 |
| Calculated Molecular Weight | 520 aa, 56 kDa |
| Observed Molecular Weight | 56 kDa |
| GenBank Accession Number | BC009001 |
| Gene Symbol | OXCT1 |
| Gene ID (NCBI) | 5019 |
| RRID | AB_2157444 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P55809 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
3-oxoacid-CoA transferase 1 (OXCT1), encoded by nuclear gene, is a mitochondrial CoA transferase required for ketone body degradation. It catalyzes the transfer of CoA from succinyl-CoA to acetoacetate, generating acetoacetyl-CoA. OXCT1 is expressed in brain, heart, and skeletal muscle, but not in liver. This antibody specifically recognizes endogenous OXCT1. (21209089)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for OXCT1 antibody 12175-1-AP | Download protocol |
| IHC protocol for OXCT1 antibody 12175-1-AP | Download protocol |
| IF protocol for OXCT1 antibody 12175-1-AP | Download protocol |
| IP protocol for OXCT1 antibody 12175-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab Hepatocyte-Macrophage Acetoacetate Shuttle Protects against Tissue Fibrosis.
| ||
Mol Cell OXCT1 succinylation and activation by SUCLA2 promotes ketolysis and liver tumor growth | ||
Cell Res Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress. | ||
Sci Adv Ketogenesis supports hepatic polyunsaturated fatty acid homeostasis via fatty acid elongation |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Kerry (Verified Customer) (09-26-2019) | Too much background. We tried all the dilutions you recommended in your literature and then more outside of both ends of that range.
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