Validation Data Gallery
|Positive WB detected in
|mouse brain tissue, HeLa cells, mouse skeletal muscle tissue, rat skeletal muscle tissue
|Positive IP detected in
|mouse brain tissue
|Positive IHC detected in
|human gliomas tissue, mouse heart tissue, mouse brain tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|HeLa cells, MCF-7 cells
|Western Blot (WB)
|WB : 1:1000-1:8000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:300-1:1200
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
12175-1-AP targets OXCT1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat, bovine
|Host / Isotype
|Rabbit / IgG
|OXCT1 fusion protein Ag2818
|3-oxoacid CoA transferase 1
|Calculated molecular weight
|520 aa, 56 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
3-oxoacid-CoA transferase 1 (OXCT1), encoded by nuclear gene, is a mitochondrial CoA transferase required for ketone body degradation. It catalyzes the transfer of CoA from succinyl-CoA to acetoacetate, generating acetoacetyl-CoA. OXCT1 is expressed in brain, heart, and skeletal muscle, but not in liver. This antibody specifically recognizes endogenous OXCT1. (21209089)
|Product Specific Protocols
|WB protocol for OXCT1 antibody 12175-1-AP
|IHC protocol for OXCT1 antibody 12175-1-AP
|IF protocol for OXCT1 antibody 12175-1-AP
|IP protocol for OXCT1 antibody 12175-1-AP
|Click here to view our Standard Protocols
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Kerry (Verified Customer) (09-26-2019)
Too much background. We tried all the dilutions you recommended in your literature and then more outside of both ends of that range.