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OXCT1 Antibody 14 Publications

Rabbit Polyclonal| Catalog number: 12175-1-AP

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Con: 38 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

OXCT1 Antibody WB mouse brain tissue 12175-1-AP

mouse brain tissue were subjected to SDS PAGE followed by western blot with 12175-1-AP( OXCT1 antibody at dilution of 1:1500 incubated at room temperature for 1.5 hours

OXCT1 Antibody IF MCF-7 cells 12175-1-AP

Immunofluorescent analysis of MCF-7 cells, using OXCT1 antibody 12175-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

OXCT1 Antibody IP mouse brain tissue 12175-1-AP

IP Result of anti-OXCT1 (IP:12175-1-AP, 3ug; Detection:12175-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

OXCT1 Antibody IHC human gliomas tissue 12175-1-AP

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12175-1-AP( OXCT1 antibody at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

OXCT1 Antibody IHC human gliomas tissue 12175-1-AP

Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12175-1-AP( OXCT1 antibody at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, mouse, rat
Positive WB detected in:
mouse brain tissue, human brain tissue, human heart tissue, mouse skeletal muscle tissue
Positive IP detected in:
mouse brain tissue
Positive IHC detected in:
human gliomas tissue, human medulloblastoma tissue, human prostate cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
MCF-7 cells
Recommended dilution:
WB : 1:500-1:3000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500
IF : 1:10-1:100
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
OXCT1 fusion protein Ag2818
Full name:
3-oxoacid CoA transferase 1
Calculated molecular weight:
520aa,56 kDa
Observed molecular weight:
56 kDa
GenBank accession number:
BC009001
Gene ID (NCBI):
5019
Gene symbol
OXCT1
Synonyms
3 oxoacid CoA transferase 1, OXCT, OXCT1, SCOT, SCOT s

Background

3-oxoacid-CoA transferase 1 (OXCT1), encoded by nuclear gene, is a mitochondrial CoA transferase required for ketone body degradation. It catalyzes the transfer of CoA from succinyl-CoA to acetoacetate, generating acetoacetyl-CoA. OXCT1 is expressed in brain, heart, and skeletal muscle, but not in liver. This antibody specifically recognizes endogenous OXCT1. (21209089)


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