Tested Applications
| Positive WB detected in | HeLa cells, mouse skeletal muscle tissue, rat brain tissue, rat skeletal muscle tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human gliomas tissue, mouse heart tissue, mouse brain tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12175-1-AP targets OXCT1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, canine, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2818 Product name: Recombinant human SCOT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 151-520 aa of BC009001 Sequence: GVPAFYTPTGYGTLVQEGGSPIKYNKDGSVAIASKPREVREFNGQHFILEEAITGDFALVKAWKADRAGNVIFRKSARNFNLPMCKAAETTVVEVEEIVDIGAFAPEDIHIPQIYVHRLIKGEKYEKRIERLSIRKEGDGEAKSAKPGDDVRERIIKRAALEFEDGMYANLGIGIPLLASNFISPNITVHLQSENGVLGLGPYPRQHEADADLINAGKETVTILPGASFFSSDESFAMIRGGHVDLTMLGAMQVSKYGDLANWMIPGKMVKGMGGAMDLVSSAKTKVVVTMEHSAKGNAHKIMEKCTLPLTGKQCVNRIITEKAVFDVDKKKGLTLIELWEGLTVDDVQKSTGCDFAVSPKLMPMQQIAN Predict reactive species |
| Full Name | 3-oxoacid CoA transferase 1 |
| Calculated Molecular Weight | 520 aa, 56 kDa |
| Observed Molecular Weight | 56 kDa |
| GenBank Accession Number | BC009001 |
| Gene Symbol | OXCT1 |
| Gene ID (NCBI) | 5019 |
| RRID | AB_2157444 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P55809 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
3-oxoacid-CoA transferase 1 (OXCT1), encoded by nuclear gene, is a mitochondrial CoA transferase required for ketone body degradation. It catalyzes the transfer of CoA from succinyl-CoA to acetoacetate, generating acetoacetyl-CoA. OXCT1 is expressed in brain, heart, and skeletal muscle, but not in liver. This antibody specifically recognizes endogenous OXCT1. (21209089)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for OXCT1 antibody 12175-1-AP | Download protocol |
| IHC protocol for OXCT1 antibody 12175-1-AP | Download protocol |
| IP protocol for OXCT1 antibody 12175-1-AP | Download protocol |
| WB protocol for OXCT1 antibody 12175-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The OXCT1 Polyclonal antibody (Cat# 12175-1-AP) has 74 citations. These appear in journals including Nature Medicine, Cell Metabolism, Cell Research, Immunity, Nature Metabolism, Molecular Cell, Nature Communications, Science Immunology, Science Advances, Oncogene, EMBO Molecular Medicine, Journal of Biological Chemistry, and American Journal of Physiology. Research fields span ketone body metabolism, ketogenic diet, cancer (hepatocellular carcinoma, glioma, lung adenocarcinoma), cardiovascular disease, neurology, immunology, and metabolic disorders such as obesity and diabetes.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther A two-strata energy flux system driven by a stress hormone prioritizes cardiac energetics | ||
J Hepatol Targeting OXCT1-mediated ketone metabolism reprograms macrophages to promote antitumor immunity via CD8+ T cells in hepatocellular carcinoma
| ||
Cell Metab Ketone flux through BDH1 supports metabolic remodeling of skeletal and cardiac muscles in response to intermittent time-restricted feeding | ||
Cell Metab Hepatocyte-Macrophage Acetoacetate Shuttle Protects against Tissue Fibrosis.
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Reviews
What customers say
The single review for this product reports excessive background in an immunohistochemistry application on mouse tissue. The reviewer noted they tested all dilutions recommended in the product literature as well as values beyond both ends of that range, yet the background issue persisted. The overall rating was low, reflecting dissatisfaction with the antibody's performance in their IHC protocol.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Kerry (Verified Customer) (09-26-2019) | Too much background. We tried all the dilutions you recommended in your literature and then more outside of both ends of that range.
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