|Positive WB detected in||mouse brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human gliomas tissue, mouse heart tissue, mouse brain tissue, human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
12175-1-AP targets OXCT1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||OXCT1 fusion protein Ag2818|
|Full Name||3-oxoacid CoA transferase 1|
|Calculated molecular weight||520 aa, 56 kDa|
|Observed molecular weight||56 kDa|
|GenBank accession number||BC009001|
|Gene ID (NCBI)||5019|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
3-oxoacid-CoA transferase 1 (OXCT1), encoded by nuclear gene, is a mitochondrial CoA transferase required for ketone body degradation. It catalyzes the transfer of CoA from succinyl-CoA to acetoacetate, generating acetoacetyl-CoA. OXCT1 is expressed in brain, heart, and skeletal muscle, but not in liver. This antibody specifically recognizes endogenous OXCT1. (21209089)
|Product Specific Protocols|
|WB protocol for OXCT1 antibody 12175-1-AP||Download protocol|
|IHC protocol for OXCT1 antibody 12175-1-AP||Download protocol|
|IF protocol for OXCT1 antibody 12175-1-AP||Download protocol|
|IP protocol for OXCT1 antibody 12175-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Ketogenic diet ameliorates axonal defects and promotes myelination in Pelizaeus-Merzbacher disease.
Biochem Biophys Res Commun
Role of OXCT1 in ovine adipose and preadipocyte differentiation.
J Lipid Res
Low ketolytic enzyme levels in tumors predict ketogenic diet responses in cancer cell lines in vitro and in vivo.
Role and therapeutic potential of dietary ketone bodies in lymph vessel growth.
E2F6 Impairs Glycolysis and Activates BDH1 Expression Prior to Dilated Cardiomyopathy.
Differential utilization of ketone bodies by neurons and glioma cell lines: a rationale for ketogenic diet as experimental glioma therapy.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Kerry (Verified Customer) (09-26-2019)
Too much background. We tried all the dilutions you recommended in your literature and then more outside of both ends of that range.