• Featured Product
  • KD/KO Validated

OXCT1 Polyclonal antibody

OXCT1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12175-1-AP

Synonyms

3 oxoacid CoA transferase 1, OXCT, OXCT1, SCOT, SCOT s



Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells, mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue, mouse heart tissue, mouse brain tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

12175-1-AP targets OXCT1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OXCT1 fusion protein Ag2818
Full Name 3-oxoacid CoA transferase 1
Calculated molecular weight 520 aa, 56 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC009001
Gene symbol OXCT1
Gene ID (NCBI) 5019
RRIDAB_2157444
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

3-oxoacid-CoA transferase 1 (OXCT1), encoded by nuclear gene, is a mitochondrial CoA transferase required for ketone body degradation. It catalyzes the transfer of CoA from succinyl-CoA to acetoacetate, generating acetoacetyl-CoA. OXCT1 is expressed in brain, heart, and skeletal muscle, but not in liver. This antibody specifically recognizes endogenous OXCT1. (21209089)

Protocols

Product Specific Protocols
WB protocol for OXCT1 antibody 12175-1-APDownload protocol
IHC protocol for OXCT1 antibody 12175-1-APDownload protocol
IF protocol for OXCT1 antibody 12175-1-APDownload protocol
IP protocol for OXCT1 antibody 12175-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Med

PMID: 25686106

Authors - Yun-Hee Youm
mouseWB

Cell Metab

PMID: 32275862

Authors - Rami Al Batran
  • KD Validated
mouseWB,IF

Cell Metab

Hepatocyte-Macrophage Acetoacetate Shuttle Protects against Tissue Fibrosis.

Authors - Patrycja Puchalska
  • KO Validated
humanWB

Cell Res

Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress.

Authors - De Huang
mouseWB,IF

Sci Adv

Ketogenic diet uncovers differential metabolic plasticity of brain cells

Authors - Tim Düking
mouseWB

Sci Immunol

Ketogenic diet activates protective γδ T cell responses against influenza virus infection.

Authors - Emily L Goldberg

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Kerry (Verified Customer) (09-26-2019)

Too much background. We tried all the dilutions you recommended in your literature and then more outside of both ends of that range.

  • Applications: Immunohistochemistry,
  • Cell Tissue Type: mouse