OXCT1 Polyclonal antibody

• Featured Product
• KD/KO Validated

OXCT1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

31

Reviews (1)

Cat no : 12175-1-AP

Synonyms

3 oxoacid CoA transferase 1, OXCT, OXCT1, SCOT, SCOT s

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Validation Data Gallery

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  WB analysis using 12175-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12175-1-AP (OXCT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12175-1-AP

  WB result of OXCT1 antibody (12175-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-OXCT1 transfected HeLa cells.

• 

  IP experiment of mouse brain using 12175-1-AP

  IP Result of anti-OXCT1 (IP:12175-1-AP, 3ug; Detection:12175-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human gliomas using 12175-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12175-1-AP (OXCT1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 12175-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12175-1-AP (OXCT1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12175-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12175-1-AP (OXCT1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 12175-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12175-1-AP (OXCT1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 12175-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12175-1-AP (OXCT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12175-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12175-1-AP (OXCT1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 12175-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using OXCT1 antibody (12175-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of MCF-7 using 12175-1-AP

  Immunofluorescent analysis of MCF-7 cells, using OXCT1 antibody 12175-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse brain tissue, HeLa cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human gliomas tissue, mouse heart tissue, mouse brain tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:300-1:1200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 7 publications below
• WB: See 30 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Product Information

12175-1-AP targets OXCT1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OXCT1 fusion protein Ag2818
• Full Name: 3-oxoacid CoA transferase 1
• Calculated molecular weight: 520 aa, 56 kDa
• Observed molecular weight: 56 kDa
• GenBank accession number: BC009001
• Gene symbol: OXCT1
• Gene ID (NCBI): 5019
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

3-oxoacid-CoA transferase 1 (OXCT1), encoded by nuclear gene, is a mitochondrial CoA transferase required for ketone body degradation. It catalyzes the transfer of CoA from succinyl-CoA to acetoacetate, generating acetoacetyl-CoA. OXCT1 is expressed in brain, heart, and skeletal muscle, but not in liver. This antibody specifically recognizes endogenous OXCT1. (21209089)

Publications

Species	Application	Title
mouse	WB	Nat Med; PMID: 25686106; Authors - Yun-Hee Youm
mouse	WB	Cell Metab; PMID: 32275862; Authors - Rami Al Batran ; KD Validated
mouse	WB,IF	Cell Metab; Hepatocyte-Macrophage Acetoacetate Shuttle Protects against Tissue Fibrosis.; Authors - Patrycja Puchalska ; KO Validated
human	WB	Cell Res; Hepatocellular carcinoma redirects to ketolysis for progression under nutrition deprivation stress.; Authors - De Huang
mouse	WB,IF	Sci Adv; Ketogenic diet uncovers differential metabolic plasticity of brain cells; Authors - Tim Düking
mouse	WB	Sci Immunol; Ketogenic diet activates protective γδ T cell responses against influenza virus infection.; Authors - Emily L Goldberg

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kerry (Verified Customer) (09-26-2019)

Too much background. We tried all the dilutions you recommended in your literature and then more outside of both ends of that range.

• Applications: Immunohistochemistry,
• Cell Tissue Type: mouse

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