SCYL2 Polyclonal antibody, PBS Only

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  WB analysis using 12325-1-AP (same clone as 12325-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12325-1-AP (SCYL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12325-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 12325-1-AP (same clone as 12325-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12325-1-AP (SCYL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12325-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse uterus using 12325-1-AP (same clone as 12325-1-PBS)

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 12325-1-AP (SCYL2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12325-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293T using 12325-1-AP (same clone as 12325-1-PBS)

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 12325-1-AP (SCYL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12325-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 12325-1-AP (same clone as 12325-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12325-1-AP (SCYL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12325-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 12325-1-AP (same clone as 12325-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12325-1-AP (SCYL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12325-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 12325-1-AP (same clone as 12325-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12325-1-AP (SCYL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12325-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 12325-1-AP (same clone as 12325-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12325-1-AP (SCYL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12325-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 12325-1-AP (same clone as 12325-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12325-1-AP (SCYL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12325-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 12325-1-AP (same clone as 12325-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12325-1-AP (SCYL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12325-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 12325-1-AP (same clone as 12325-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12325-1-AP (SCYL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12325-1-PBS in a different storage buffer formulation.

SCYL2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 12325-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

12325-1-PBS targets SCYL2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2988

  Product name: Recombinant human SCYL2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 157-505 aa of BC012387

  Sequence: NNLNLNQFNSFISVIKEMLNRLESEHKTKLEQLHIMQEQQKSLDIGNQMNVSEEMKVINIGNQQIDKVFNNIGADLLTGSESENKEDGLQNKHKRASLTLEEKQKLAKEQEQAQKLKSQQPLKPQVHTPVATVKQTKDLTDTLMDNMSSLTSLSVSTPKSSASSTFTSVPSMGIGMMFSTPTDNTKRNLTNGLNANMGFQTSGFNMPVNTNQNFYSSPSTVGVTKMTLGTPPTLPNFNALSVPPAGAKQTQQRPTDMSALNNLFGPQKPKVSMNQLSQQKPNQWLNQFVPPQGSPTMGSSVMGTQMNVIGQSAFGMQGNPFFNPQNFAQPPTTMTNSSSASNDLKDLFG

• Full Name: SCY1-like 2 (S. cerevisiae)
• Calculated Molecular Weight: 929 aa, 104 kDa
• Observed Molecular Weight: 104 kDa
• GenBank Accession Number: BC012387
• Gene Symbol: SCYL2
• Gene ID (NCBI): 55681
• RRID: AB_2071019
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6P3W7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.