SCYL2 antibody (12325-1-PBS)

Filter: AllWBIHC

WB analysis using 12325-1-AP (same clone as 12325-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 12325-1-AP (SCYL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12325-1-PBS in a different storage buffer formulation.
WB analysis of mouse uterus using 12325-1-AP (same clone as 12325-1-PBS)
mouse uterus tissue were subjected to SDS PAGE followed by western blot with 12325-1-AP (SCYL2 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12325-1-PBS in a different storage buffer formulation.
WB analysis of NIH/3T3 using 12325-1-AP (same clone as 12325-1-PBS)
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12325-1-AP (SCYL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12325-1-PBS in a different storage buffer formulation.
WB analysis of Jurkat using 12325-1-AP (same clone as 12325-1-PBS)
Jurkat cells were subjected to SDS PAGE followed by western blot with 12325-1-AP (SCYL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12325-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293T using 12325-1-AP (same clone as 12325-1-PBS)
HEK-293T cells were subjected to SDS PAGE followed by western blot with 12325-1-AP (SCYL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12325-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 12325-1-AP (same clone as 12325-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 12325-1-AP (SCYL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12325-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 12325-1-AP (same clone as 12325-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 12325-1-AP (SCYL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12325-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 12325-1-AP (same clone as 12325-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12325-1-AP (SCYL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12325-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 12325-1-AP (same clone as 12325-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12325-1-AP (SCYL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12325-1-PBS in a different storage buffer formulation.
IHC staining of mouse cerebellum using 12325-1-AP (same clone as 12325-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12325-1-AP (SCYL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12325-1-PBS in a different storage buffer formulation.
IHC staining of mouse cerebellum using 12325-1-AP (same clone as 12325-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12325-1-AP (SCYL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12325-1-PBS in a different storage buffer formulation.

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Product Information

12325-1-PBS targets SCYL2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SCYL2 fusion protein Ag2988 Predict reactive species
Full Name	SCY1-like 2 (S. cerevisiae)
Calculated Molecular Weight	929 aa, 104 kDa
Observed Molecular Weight	104 kDa
GenBank Accession Number	BC012387
Gene Symbol	SCYL2
Gene ID (NCBI)	55681
RRID	AB_2071019
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q6P3W7
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.