SCYL2 Polyclonal antibody

SCYL2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12325-1-AP

Synonyms

CVAK104, KIAA1360, SCY1 like 2 (S. cerevisiae), SCY1 like protein 2, SCYL2

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Validation Data Gallery

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  WB analysis using 12325-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12325-1-AP (SCYL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 12325-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12325-1-AP (SCYL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 12325-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 12325-1-AP (SCYL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12325-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12325-1-AP (SCYL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12325-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12325-1-AP (SCYL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12325-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12325-1-AP (SCYL2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse uterus using 12325-1-AP

  mouse uterus tissue were subjected to SDS PAGE followed by western blot with 12325-1-AP (SCYL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 12325-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12325-1-AP (SCYL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12325-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12325-1-AP (SCYL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 12325-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12325-1-AP (SCYL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 12325-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12325-1-AP (SCYL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293T cells, NIH/3T3 cells, mouse uterus tissue, Jurkat cells, HeLa cells, HepG2 cells
• Positive IHC detected in: mouse brain tissue, mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 2 publications below

Product Information

12325-1-AP targets SCYL2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SCYL2 fusion protein Ag2988
• Full Name: SCY1-like 2 (S. cerevisiae)
• Calculated Molecular Weight: 929 aa, 104 kDa
• Observed Molecular Weight: 104 kDa
• GenBank Accession Number: BC012387
• Gene Symbol: SCYL2
• Gene ID (NCBI): 55681
• RRID: AB_2071019
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	J Neurosci; SCYL2 Protects CA3 Pyramidal Neurons from Excitotoxicity during Functional Maturation of the Mouse Hippocampus.; Authors - Sebastien Gingras
human	WB,IF	J Cell Biochem; Phosphorylated LASS2 inhibits prostate carcinogenesis via negative regulation of Wnt/β-catenin signaling.; Authors - Kuangen Zhang