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SEC5/EXOC2 Polyclonal antibody
SEC5/EXOC2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 12751-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, human brain tissue, human ileum tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 8 publications below |
WB | See 17 publications below |
IHC | See 1 publications below |
IF | See 7 publications below |
IP | See 1 publications below |
CoIP | See 1 publications below |
Product Information
12751-1-AP targets SEC5/EXOC2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | SEC5/EXOC2 fusion protein Ag3147 |
Full Name | exocyst complex component 2 |
Calculated Molecular Weight | 924 aa, 104 kDa |
Observed Molecular Weight | 95-100 kDa |
GenBank Accession Number | BC016918 |
Gene Symbol | SEC5 |
Gene ID (NCBI) | 55770 |
RRID | AB_2262528 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
EXOC2 (exocyst complex component 2), also known as SEC5 and SEC5L1, is a component of the exocyst complex, and is required to mediate RalB-dependent survival signals in transformed cells. The exocyst complex, composed of eight evolutionarily conserved subunits (SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70, and EXO84), is involved in tethering post-Golgi secretory vesicles to specific plasma membrane domains. The gene of EXOC2 maps to chromosome 6p25.3, and encodes a 924-amino acid protein with an experimentally determined molecular mass of 95-100 kDa. EXOC2 mRNA is widely expressed with highest levels in brain and placenta.
Protocols
Product Specific Protocols | |
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WB protocol for SEC5/EXOC2 antibody 12751-1-AP | Download protocol |
IHC protocol for SEC5/EXOC2 antibody 12751-1-AP | Download protocol |
IF protocol for SEC5/EXOC2 antibody 12751-1-AP | Download protocol |
IP protocol for SEC5/EXOC2 antibody 12751-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Cell Biol M-Sec promotes membrane nanotube formation by interacting with Ral and the exocyst complex.
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Nat Cell Biol The deubiquitylase USP33 discriminates between RALB functions in autophagy and innate immune response.
| ||
Blood vWF maturation and release are controlled by two regulators of Weibel-Palade body biogenesis: exocyst and BLOC-2. | ||
Sci Signal Phosphorylation of the exocyst protein Exo84 by TBK1 promotes insulin-stimulated GLUT4 trafficking. |