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  • KD/KO Validated

SEC5/EXOC2 Polyclonal antibody

SEC5/EXOC2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12751-1-AP

Synonyms

EXOC2, exocyst complex component 2, Exocyst complex component Sec5, SEC5, SEC5L1, Sec5p



Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, human ileum tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12751-1-AP targets SEC5/EXOC2 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SEC5/EXOC2 fusion protein Ag3147
Full Name exocyst complex component 2
Calculated Molecular Weight 924 aa, 104 kDa
Observed Molecular Weight 95-100 kDa
GenBank Accession NumberBC016918
Gene Symbol EXOC2
Gene ID (NCBI) 55770
RRIDAB_2262528
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EXOC2 (exocyst complex component 2), also known as SEC5 and SEC5L1, is a component of the exocyst complex, and is required to mediate RalB-dependent survival signals in transformed cells. The exocyst complex, composed of eight evolutionarily conserved subunits (SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70, and EXO84), is involved in tethering post-Golgi secretory vesicles to specific plasma membrane domains. The gene of EXOC2 maps to chromosome 6p25.3, and encodes a 924-amino acid protein with an experimentally determined molecular mass of 95-100 kDa. EXOC2 mRNA is widely expressed with highest levels in brain and placenta.

Protocols

Product Specific Protocols
WB protocol for SEC5/EXOC2 antibody 12751-1-APDownload protocol
IHC protocol for SEC5/EXOC2 antibody 12751-1-APDownload protocol
IF protocol for SEC5/EXOC2 antibody 12751-1-APDownload protocol
IP protocol for SEC5/EXOC2 antibody 12751-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

A kinase-independent role for EGF receptor in autophagy initiation.

Authors - Xiaojun Tan
  • KD Validated
humanWB

Nat Cell Biol

M-Sec promotes membrane nanotube formation by interacting with Ral and the exocyst complex.

Authors - Hase Koji K
  • KD Validated
humanWB,IF

Nat Cell Biol

The deubiquitylase USP33 discriminates between RALB functions in autophagy and innate immune response.

Authors - Simicek Michal M
  • KD Validated
humanWB

Blood

vWF maturation and release are controlled by two regulators of Weibel-Palade body biogenesis: exocyst and BLOC-2.

Authors - Anish Vaibhav Sharda
mouseWB,IP

Mol Cell

The anti-inflammatory TIPE2 is an inhibitor of the oncogenic Ras.

Authors - Gus-Brautbar Yael Y
mouseWB,CoIP

Sci Signal

Phosphorylation of the exocyst protein Exo84 by TBK1 promotes insulin-stimulated GLUT4 trafficking.

Authors - Maeran Uhm