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- KD/KO Validated
SEC5/EXOC2 Polyclonal antibody
SEC5/EXOC2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Cat no : 12751-1-AP
|Positive WB detected in||mouse brain tissue, human brain tissue, human ileum tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12751-1-AP targets SEC5/EXOC2 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SEC5/EXOC2 fusion protein Ag3147|
|Full Name||exocyst complex component 2|
|Calculated molecular weight||924 aa, 104 kDa|
|Observed molecular weight||95-100 kDa|
|GenBank accession number||BC016918|
|Gene ID (NCBI)||55770|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
EXOC2 (exocyst complex component 2), also known as SEC5 and SEC5L1, is a component of the exocyst complex, and is required to mediate RalB-dependent survival signals in transformed cells. The exocyst complex, composed of eight evolutionarily conserved subunits (SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70, and EXO84), is involved in tethering post-Golgi secretory vesicles to specific plasma membrane domains. The gene of EXOC2 maps to chromosome 6p25.3, and encodes a 924-amino acid protein with an experimentally determined molecular mass of 95-100 kDa. EXOC2 mRNA is widely expressed with highest levels in brain and placenta.
|Product Specific Protocols|
|WB protocol for SEC5/EXOC2 antibody 12751-1-AP||Download protocol|
|IHC protocol for SEC5/EXOC2 antibody 12751-1-AP||Download protocol|
|IF protocol for SEC5/EXOC2 antibody 12751-1-AP||Download protocol|
|IP protocol for SEC5/EXOC2 antibody 12751-1-AP||Download protocol|
|Click here to view our Standard Protocols|
A kinase-independent role for EGF receptor in autophagy initiation.
Nat Cell Biol
M-Sec promotes membrane nanotube formation by interacting with Ral and the exocyst complex.
Nat Cell Biol
The deubiquitylase USP33 discriminates between RALB functions in autophagy and innate immune response.
vWF maturation and release are controlled by two regulators of Weibel-Palade body biogenesis: exocyst and BLOC-2.
The anti-inflammatory TIPE2 is an inhibitor of the oncogenic Ras.
Phosphorylation of the exocyst protein Exo84 by TBK1 promotes insulin-stimulated GLUT4 trafficking.