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SEC5/EXOC2 Monoclonal antibody
SEC5/EXOC2 Monoclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 66011-1-Ig
Validation Data Gallery
|Positive WB detected in||mouse brain tissue, pig brain tissue, human brain tissue, pig cerebellum tissue, rat brain tissue, rat cerebellum tissue, HEK-293 cells|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||human breast cancer tissue, human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells, MDA-MB-231 cells|
|Western Blot (WB)||WB : 1:2500-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:200-1:800|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
66011-1-Ig targets SEC5/EXOC2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, pig, rat samples.
|Tested Reactivity||human, mouse, pig, rat|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||SEC5/EXOC2 fusion protein Ag17866|
|Full Name||exocyst complex component 2|
|Calculated molecular weight||924 aa, 104 kDa|
|Observed molecular weight||100 kDa|
|GenBank accession number||BC016918|
|Gene ID (NCBI)||55770|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
EXOC2 (exocyst complex component 2), also known as SEC5 and SEC5L1, is a component of the exocyst complex, and is required to mediate RalB-dependent survival signals in transformed cells. The exocyst complex, composed of eight evolutionarily conserved subunits (SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70, and EXO84), is involved in tethering post-Golgi secretory vesicles to specific plasma membrane domains. The gene of EXOC2 maps to chromosome 6p25.3, and encodes a 924-amino acid protein with an experimentally determined molecular mass of 95-100 kDa. EXOC2 mRNA is widely expressed with highest levels in brain and placenta.
|Product Specific Protocols|
|WB protocol for SEC5/EXOC2 antibody 66011-1-Ig||Download protocol|
|IHC protocol for SEC5/EXOC2 antibody 66011-1-Ig||Download protocol|
|IF protocol for SEC5/EXOC2 antibody 66011-1-Ig||Download protocol|
|IP protocol for SEC5/EXOC2 antibody 66011-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
Intercellular nanotubes mediate mitochondrial trafficking between cancer and immune cells.
Shigella hijacks the exocyst to cluster macropinosomes for efficient vacuolar escape.
J Cell Sci
Dexamethasone-induced cellular tension requires SGK1-stimulated Sec5/GEF-H1 interaction.
Int J Cancer
Myoferlin plays a key role in VEGFA secretion and impacts tumor-associated angiogenesis in human pancreas cancer.
Exocyst complex protein expression in the human placenta.