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  • KD/KO Validated

SEC5/EXOC2 Monoclonal antibody

SEC5/EXOC2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, pig, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

1C11G2

Cat no : 66011-1-Ig

Synonyms

EXOC2, exocyst complex component 2, Exocyst complex component Sec5, SEC5, SEC5/EXOC2, SEC5L1, Sec5p



Tested Applications

Positive WB detected inmouse brain tissue, pig brain tissue, human brain tissue, pig cerebellum tissue, rat brain tissue, rat cerebellum tissue, HEK-293 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, MDA-MB-231 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2500-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66011-1-Ig targets SEC5/EXOC2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, pig, rat samples.

Tested Reactivity human, mouse, pig, rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SEC5/EXOC2 fusion protein Ag17866
Full Name exocyst complex component 2
Calculated Molecular Weight 924 aa, 104 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC016918
Gene Symbol SEC5
Gene ID (NCBI) 55770
RRIDAB_11042317
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EXOC2 (exocyst complex component 2), also known as SEC5 and SEC5L1, is a component of the exocyst complex, and is required to mediate RalB-dependent survival signals in transformed cells. The exocyst complex, composed of eight evolutionarily conserved subunits (SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70, and EXO84), is involved in tethering post-Golgi secretory vesicles to specific plasma membrane domains. The gene of EXOC2 maps to chromosome 6p25.3, and encodes a 924-amino acid protein with an experimentally determined molecular mass of 95-100 kDa. EXOC2 mRNA is widely expressed with highest levels in brain and placenta.

Protocols

Product Specific Protocols
WB protocol for SEC5/EXOC2 antibody 66011-1-IgDownload protocol
IHC protocol for SEC5/EXOC2 antibody 66011-1-IgDownload protocol
IF protocol for SEC5/EXOC2 antibody 66011-1-IgDownload protocol
IP protocol for SEC5/EXOC2 antibody 66011-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Nat Nanotechnol

Intercellular nanotubes mediate mitochondrial trafficking between cancer and immune cells.

Authors - Tanmoy Saha
  • KD Validated
humanIF

PLoS Pathog

Shigella hijacks the exocyst to cluster macropinosomes for efficient vacuolar escape.

Authors - Yuen-Yan Chang
human

J Cell Sci

Dexamethasone-induced cellular tension requires SGK1-stimulated Sec5/GEF-H1 interaction.

Authors - Hong-Ling Wang
humanIF

Int J Cancer

Myoferlin plays a key role in VEGFA secretion and impacts tumor-associated angiogenesis in human pancreas cancer.

Authors - Karim Fahmy
humanWB,IF

Placenta

Exocyst complex protein expression in the human placenta.

Authors - I M Gonzalez
mouseWB

Commun Biol

The exocyst complex and intracellular vesicles mediate soluble protein trafficking to the primary cilium

Authors - S M Niedziółka