CoraLite®594-conjugated SEC5/EXOC2 Monoclonal antibody

SEC5/EXOC2 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, pig, rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1C11G2

Cat no : CL594-66011

Synonyms

EXOC2, exocyst complex component 2, Exocyst complex component Sec5, FLJ11026, SEC5, SEC5L1, Sec5p

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Validation Data Gallery

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  IF Staining of HeLa using CL594-66011

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL594-66011 (SEC5/EXOC2 antibody) at dilution of 1:100.

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Tested Applications

• Positive IF detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of CL594-66011 is SEC5/EXOC2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, pig, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: SEC5/EXOC2 fusion protein Ag17866
• Full Name: exocyst complex component 2
• Calculated Molecular Weight: 924 aa, 104 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC016918
• Gene Symbol: EXOC2
• Gene ID (NCBI): 55770
• RRID: AB_2883476
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EXOC2 (exocyst complex component 2), also known as SEC5 and SEC5L1, is a component of the exocyst complex, and is required to mediate RalB-dependent survival signals in transformed cells. The exocyst complex, composed of eight evolutionarily conserved subunits (SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70, and EXO84), is involved in tethering post-Golgi secretory vesicles to specific plasma membrane domains. The gene of EXOC2 maps to chromosome 6p25.3, and encodes a 924-amino acid protein with an experimentally determined molecular mass of 95-100 kDa. EXOC2 mRNA is widely expressed with highest levels in brain and placenta.