SEL1L Recombinant monoclonal antibody, PBS Only (Capture)

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  Sandwich ELISA sample test of MP01186-1

  The mean SEL1L concentration was determined to be 243.66 ng/mL in HepG2 cell extract based on a 2.30 mg/mL extract load, 142.85 ng/mL in A549 cell extract based on a 2.70 mg/mL extract load and 66.47 ng/mL in mouse kidney tissue extract based on a 3.10 mg/mL extract load.

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  Sandwich ELISA standard curve of MP01186-1

  Sandwich ELISA standard curve of MP01186-1, Human SEL1L Recombinant Matched Antibody Pair - PBS only. 84317-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag32178. 84317-2-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

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  Cytometric bead array standard curve of MP01186-1

  Cytometric bead array standard curve of MP01186-1, SEL1L Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84317-5-PBS. Detection antibody: 84317-2-PBS. Standard: Ag32178. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 84317-5-PBS

  Biolayer interferometry (BLl) kinetic assay of 84317-5-PBS against Human SEL1L was performed. The affinity constant is 2.03 nM.

SEL1L Uni-rAb^® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA

Cat No. 84317-5-PBS

Clone No.241594H5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

Protein sel 1 homolog 1, Protein sel-1 homolog 1, Sel 1L, Sel-1L, Suppressor of lin-12-like protein 1

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Product Information

84317-5-PBS targets SEL1L as part of a matched antibody pair:

MP01186-1: 84317-5-PBS capture and 84317-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag32178

  Product name: Recombinant human SEL1L protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 22-225 aa of NM_005065

  Sequence: SSDEEGSQDESLDSKTTLTSDESVKDHTTAGRVVAGQIFLDSEESELESSIQEEEDSLKSQEGESVTEDISFLESPNPENKDYEEPKKVRKPALTAIEGTAHGEPCHFPFLFLDKEYDECTSDGREDGRLWCATTYDYKADEKWGFCETEEEAAKRRQMQEAEMMYQTGMKILNGSNKKSQKREAYRYLQKAASMNHTKALERV

• Full Name: sel-1 suppressor of lin-12-like (C. elegans)
• Calculated Molecular Weight: 89 kDa
• GenBank Accession Number: NM_005065
• Gene Symbol: SEL1L
• Gene ID (NCBI): 6400
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UBV2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.