Validation Data Gallery
|Positive WB detected in||HuH-7 cells, PC-12 cells, HepG2 cells, human placenta tissue, U2OS cells|
|Positive IP detected in||HepG2 cells|
|Positive IHC detected in||human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HUVEC cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
13801-1-AP targets PAI-1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, bovine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PAI-1 fusion protein Ag4777|
|Full Name||serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1|
|Calculated molecular weight||402 aa, 45 kDa|
|Observed molecular weight||45 kDa|
|GenBank accession number||BC010860|
|Gene ID (NCBI)||5054|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
SERPINE1, also named as Plasminogen activator inhibitor type 1 (PAI-1), is a member of the serine protease inhibitor (SERPIN) superfamily. PAI-1 is the principal inhibitor of tPA and uPA, and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. In western blotting, we got two bands 50 kDa and 90-110 kDa, and 90-110 kDa consistent with PAI-1 in complex with its target protease, t-PA (PMID: 21596853).
|Product Specific Protocols|
|WB protocol for PAI-1 antibody 13801-1-AP||Download protocol|
|IHC protocol for PAI-1 antibody 13801-1-AP||Download protocol|
|IF protocol for PAI-1 antibody 13801-1-AP||Download protocol|
|IP protocol for PAI-1 antibody 13801-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Cell Physiol
Knockdown of TRIM27 expression suppresses the dysfunction of mesangial cells in lupus nephritis by FoxO1 pathway.
Biomed Res Int
Silencing of ELK3 Induces S-M Phase Arrest and Apoptosis and Upregulates SERPINE1 Expression Reducing Migration in Prostate Cancer Cells.
Acta Pharmacol Sin
Polydatin attenuates renal fibrosis in diabetic mice through regulating the Cx32-Nox4 signaling pathway.
E4 engages uPAR and enolase-1 and activates urokinase to exert antifibrotic effects.
Cell Death Dis
SIX5-activated LINC01468 promotes lung adenocarcinoma progression by recruiting SERBP1 to regulate SERPINE1 mRNA stability and recruiting USP5 to facilitate PAI1 protein deubiquitylation.
Mesangial cell-derived tenascin-C contributes to mesangial cell proliferation and matrix protein production in IgA nephropathy.