PAI-1 Polyclonal antibody

PAI-1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, rat and More (1)





Cat no : 13801-1-AP


PAI, PAI 1, PAI1, PAI-1, PLANH1, Serpin E1, SERPINE1, PAI-1

Tested Applications

Positive WB detected inHuH-7 cells, HepG2 cells, PC-12 cells, U2OS cells, human placenta tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHUVEC cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13801-1-AP targets PAI-1 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Cited Reactivityhuman, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PAI-1 fusion protein Ag4777
Full Name serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
Calculated Molecular Weight 402 aa, 45 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC010860
Gene Symbol SERPINE1
Gene ID (NCBI) 5054
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SERPINE1, also named as Plasminogen activator inhibitor type 1 (PAI-1), is a member of the serine protease inhibitor (SERPIN) superfamily. PAI-1 is the principal inhibitor of tPA and uPA, and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. In western blotting, we got two bands 50 kDa and 90-110 kDa, and 90-110 kDa consistent with PAI-1 in complex with its target protease, t-PA (PMID: 21596853).


Product Specific Protocols
WB protocol for PAI-1 antibody 13801-1-APDownload protocol
IHC protocol for PAI-1 antibody 13801-1-APDownload protocol
IF protocol for PAI-1 antibody 13801-1-APDownload protocol
IP protocol for PAI-1 antibody 13801-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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Nat Commun

A translational program that suppresses metabolism to shield the genome.

Authors - Nathan C Balukoff

Nat Commun

ICAM1 initiates CTC cluster formation and trans-endothelial migration in lung metastasis of breast cancer.

Authors - Rokana Taftaf

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A network of RNA-binding proteins controls translation efficiency to activate anaerobic metabolism.

Authors - J J David Ho


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


AIKATERINI (Verified Customer) (03-15-2023)

The antibody was diluted in TBS-T 5% non fat milk.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Hela
PAI-1 Antibody Western Blot validation (1:1000 dilution) in Hela (Cat no:13801-1-AP)