|Positive WB detected in||HuH-7 cells, PC-12 cells, HepG2 cells, human placenta tissue, U2OS cells|
|Positive IHC detected in||human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
13801-1-AP targets PAI-1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||bovine, human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PAI-1 fusion protein Ag4777|
|Full Name||serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1|
|Calculated molecular weight||402 aa, 45 kDa|
|Observed molecular weight||45 kDa|
|GenBank accession number||BC010860|
|Gene ID (NCBI)||5054|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
SERPINE1, also named as Plasminogen activator inhibitor type 1 (PAI-1), is a member of the serine protease inhibitor (SERPIN) superfamily. PAI-1 is the principal inhibitor of tPA and uPA, and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. In western blotting, we got two bands 50 kDa and 90-110 kDa, and 90-110 kDa consistent with PAI-1 in complex with its target protease, t-PA (PMID: 21596853).
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