PAI-1 Polyclonal antibody

PAI-1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

45

Reviews (1)

Cat no : 13801-1-AP

Synonyms

PAI, PAI 1, PAI1, PAI-1, PLANH1, Serpin E1, SERPINE1, PAI-1

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Validation Data Gallery

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  WB analysis using 13801-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13801-1-AP (PAI-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13801-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13801-1-AP (PAI-1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13801-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13801-1-AP (PAI-1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13801-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13801-1-AP (PAI-1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 13801-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 13801-1-AP (PAI-1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 13801-1-AP

  IP result of anti-PAI-1 (IP:13801-1-AP, 4ug; Detection:13801-1-AP 1:2000) with HepG2 cells lysate 1360 ug.

• 

  IHC staining of human lung cancer using 13801-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13801-1-AP (PAI-1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 13801-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 13801-1-AP (PAI-1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HUVEC using 13801-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HUVEC cells using PAI-1 antibody (13801-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HuH-7 cells, HepG2 cells, PC-12 cells, U2OS cells, human placenta tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 41 publications below
• IHC: See 9 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Product Information

13801-1-AP targets PAI-1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PAI-1 fusion protein Ag4777
• Full Name: serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
• Calculated Molecular Weight: 402 aa, 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC010860
• Gene Symbol: SERPINE1
• Gene ID (NCBI): 5054
• RRID: AB_2186881
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SERPINE1, also named as Plasminogen activator inhibitor type 1 (PAI-1), is a member of the serine protease inhibitor (SERPIN) superfamily. PAI-1 is the principal inhibitor of tPA and uPA, and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. In western blotting, we got two bands 50 kDa and 90-110 kDa, and 90-110 kDa consistent with PAI-1 in complex with its target protease, t-PA (PMID: 21596853).

Publications

Species	Application	Title
human	WB	Am J Respir Crit Care Med; CD38 Mediates Lung Fibrosis by Promoting Alveolar Epithelial Cell Aging; Authors - Huachun Cui
human	WB	Cancer Discov; Dynamic glycoprotein hyposialylation promotes chemotherapy evasion and metastatic seeding of quiescent circulating tumor cell clusters in breast cancer; Authors - Nurmaa K Dashzeveg
human	CoIP,WB,IHC	Circ Res; MiR-18a inhibits BMP4 and HIF-1α Normalizing Brain Arteriovenous Malformations.; Authors - Nagore I Marín-Ramos
human	WB,IHC,IF	Nat Commun; A translational program that suppresses metabolism to shield the genome.; Authors - Nathan C Balukoff
human	WB	Nat Commun; ICAM1 initiates CTC cluster formation and trans-endothelial migration in lung metastasis of breast cancer.; Authors - Rokana Taftaf
human	WB	Nat Commun; A network of RNA-binding proteins controls translation efficiency to activate anaerobic metabolism.; Authors - J J David Ho

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

AIKATERINI (Verified Customer) (03-15-2023)

The antibody was diluted in TBS-T 5% non fat milk.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hela

PAI-1 Antibody Western Blot validation (1:1000 dilution) in Hela (Cat no:13801-1-AP)

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