Tested Applications
| Positive WB detected in | HepG2 cells, HuH-7 cells, L02 cells, MG-63 cells, U2OS cells, human placenta tissue |
| Positive IP detected in | HUVEC cells |
| Positive IHC detected in | human placenta tissue, human lung cancer tissue, human malignant melanoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse kidney tissue |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13801-1-AP targets Serpin E1/PAI-1 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA, Blocking assay applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, rabbit, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4777 Product name: Recombinant human SERPINE1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-402 aa of BC010860 Sequence: MQMSPALTCLVLGLALVFGEGSAVHHPPSYVAHLASDFGVRVFQQVAQASKDRNVVFSPYGVASVLAMLQLTTGGETQQQIQAAMGFKIDDKGMAPALRHLYKELMGPWNKDEISTTDAIFVQRDLKLVQGFMPHFFRLFRSTVKQVDFSEVERARFIINDWVKTHTKGMISNLLGKGAVDQLTRLVLVNALYFNGQWKTPFPDSSTHRRLFHKSDGSTVSVPMMAQTNKFNYTEFTTPDGHYYDILELPYHGDTLSMFIAAPYEKEVPLSALTNILSAQLISHWKGNMTRLPRLLVLPKFSLETEVDLRKPLENLGMTDMFRQFQADFTSLSDQEPLHVAQALQKVKIEVNESGTVASSSTAVIVSARMAPEEIIMDRPFLFVVRHNPTGTVLFMGQVMEP Predict reactive species |
| Full Name | serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 |
| Calculated Molecular Weight | 402 aa, 45 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC010860 |
| Gene Symbol | PAI-1 |
| Gene ID (NCBI) | 5054 |
| RRID | AB_2186881 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P05121 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SERPINE1, also named as Plasminogen activator inhibitor type 1 (PAI-1), is a member of the serine protease inhibitor (SERPIN) superfamily. PAI-1 is the principal inhibitor of tPA and uPA, and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. In western blotting, we got two bands 50 kDa and 90-110 kDa, and 90-110 kDa consistent with PAI-1 in complex with its target protease, t-PA (PMID: 21596853).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Serpin E1/PAI-1 antibody 13801-1-AP | Download protocol |
| IHC protocol for Serpin E1/PAI-1 antibody 13801-1-AP | Download protocol |
| IP protocol for Serpin E1/PAI-1 antibody 13801-1-AP | Download protocol |
| WB protocol for Serpin E1/PAI-1 antibody 13801-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Serpin E1/PAI-1 Polyclonal antibody (Cat# 13801-1-AP) has accumulated 107 citations across journals including Nature Communications, Cancer Discovery, Cell Stem Cell, Science Advances, EMBO Journal, and PNAS. Research fields span oncology (breast, lung, gastric, liver cancers), fibrosis (pulmonary, renal, hepatic), cardiovascular disease (atherosclerosis, ARDS, thrombosis), diabetes, neurology, aging/senescence, and inflammation. Applications are predominantly Western blot, with IHC, IF, and Co-IP also represented.
| Species | Application | Title |
|---|---|---|
Cancer Discov Dynamic glycoprotein hyposialylation promotes chemotherapy evasion and metastatic seeding of quiescent circulating tumor cell clusters in breast cancer | ||
Am J Respir Crit Care Med CD38 Mediates Lung Fibrosis by Promoting Alveolar Epithelial Cell Aging | ||
Nat Commun A network of RNA-binding proteins controls translation efficiency to activate anaerobic metabolism. | ||
Nat Commun A circular RNA activated by TGFβ promotes tumor metastasis through enhancing IGF2BP3-mediated PDPN mRNA stability |
Reviews
What customers say
The single review for this antibody rates it 4 out of 5 stars. The user performed a Western Blot on HeLa cells using a 1:1000 dilution in TBS-T with 5% non-fat milk, and reported a positive result. Overall, the feedback is favorable, indicating the antibody performed well under standard WB conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
AIKATERINI (Verified Customer) (03-15-2023) | The antibody was diluted in TBS-T 5% non fat milk.
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