SESN1 Polyclonal antibody

SESN1 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21668-1-AP

Synonyms

p53 regulated protein PA26, PA26, RP11 787I22.1, SESN1, SEST1, sestrin 1



Tested Applications

Positive WB detected inhuman skeletal muscle tissue, human testis tissue, mouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21668-1-AP is SESN1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SESN1 fusion protein Ag16462
Full Name sestrin 1
Calculated molecular weight 551 aa, 64 kDa
Observed molecular weight 66-68 kDa
GenBank accession numberBC112036
Gene symbol SESN1
Gene ID (NCBI) 27244
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 1 is implicated in the inhibition of cell growth. It is approximately a 66kDa protein in humans (551 amino acids).

Protocols

Product Specific Protocols
WB protocol for SESN1 antibody 21668-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Am J Physiol Endocrinol Metab

Evidence for a Role for Sestrin1 in Mediating Leucine-Induced Activation of mTORC1 in Skeletal Muscle.

Authors - Dandan Xu
mouseWB

Biochim Biophys Acta

Exercise improves glucose uptake in murine myotubes through the AMPKα2-mediated induction of Sestrins.

Authors - Tianyi Wang
mouseWB

Physiol Rep

Repressors of mTORC1 act to blunt the anabolic response to feeding in the soleus muscle of a cast-immobilized mouse hindlimb.

Authors - Kevin L Shimkus
mouseWB

Biomed Res Int

Pentamethylquercetin Attenuates Cardiac Remodeling via Activation of the Sestrins/Keap1/Nrf2 Pathway in MSG-Induced Obese Mice.

Authors - Jingxia Du
humanWB,IHC

Cell Physiol Biochem

Sestrin-Mediated Inhibition of Stress-Induced Intervertebral Disc Degradation Through the Enhancement of Autophagy.

Authors - Ji Tu
  • KD Validated
mouseWB

Appl Physiol Nutr Metab

Exercise improves lipid metabolism disorders induced by high-fat diet in a SESN2/JNK- independent manner.

Authors - Tianyi Wang