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SESN3 Polyclonal antibody

SESN3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24532-1-AP

Synonyms

SESN3, SEST3, sestrin 3



Tested Applications

Positive WB detected inHepG2 cells, A431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24532-1-AP is SESN3 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SESN3 fusion protein Ag21380
Full Name sestrin 3
Calculated molecular weight 492 aa, 57 kDa
Observed molecular weight 57 kDa
GenBank accession numberBC017296
Gene symbol SESN3
Gene ID (NCBI) 143686
RRIDAB_2879593
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 3 was identified as a forkhead box O (FoxO) target gene with antioxidant activity. Recently it was reported that sestrin 3 may play an important role in AKT induced increase in ROS and it might be a promising target in selectively killing cancer cells containing high levels of AKT activity.

Protocols

Product Specific Protocols
WB protocol for SESN3 antibody 24532-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

J Orthop Res

Age-related reduction in the expression of FOXO transcription factors and correlations with intervertebral disc degeneration.

Authors - Oscar Alvarez-Garcia
humanWB

Mol Cell

Ring domains are essential for GATOR2-dependent mTORC1 activation

Authors - Cong Jiang