SESN3 Polyclonal antibody

• Featured Product
• KD/KO Validated

SESN3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 24532-1-AP

Synonyms

SESN3, SEST3, sestrin 3

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Validation Data Gallery

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  WB analysis of A431 using 24532-1-AP

  WB result of SESN3 antibody (24532-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SESN3 transfected A431 cells.

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  WB analysis of HepG2 using 24532-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24532-1-AP (SESN3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HepG2 cells, A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 24532-1-AP is SESN3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SESN3 fusion protein Ag21380
• Full Name: sestrin 3
• Calculated molecular weight: 492 aa, 57 kDa
• Observed molecular weight: 57 kDa
• GenBank accession number: BC017296
• Gene symbol: SESN3
• Gene ID (NCBI): 143686
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 3 was identified as a forkhead box O (FoxO) target gene with antioxidant activity. Recently it was reported that sestrin 3 may play an important role in AKT induced increase in ROS and it might be a promising target in selectively killing cancer cells containing high levels of AKT activity.

Publications

Species	Application	Title
human	IHC	J Orthop Res; Age-related reduction in the expression of FOXO transcription factors and correlations with intervertebral disc degeneration.; Authors - Oscar Alvarez-Garcia
human	WB	Mol Cell; Ring domains are essential for GATOR2-dependent mTORC1 activation; Authors - Cong Jiang