SETD1B Polyclonal antibody

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• KD/KO Validated

SETD1B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 55005-1-AP Copied!

Publications (11)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Synonyms

EC:2.1.1.364, Histone-lysine N-methyltransferase SETD1B, hSET1B, KIAA1076, KMT2G

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  WB analysis of human brain using 55005-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55005-1-AP (SETD1B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis of mouse liver using 55005-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55005-1-AP (SETD1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse liver using 55005-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 55005-1-AP (SETD1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 55005-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 55005-1-AP (SETD1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IP experiment of MCF-7 using 55005-1-AP

  IP result of anti-SETD1B (IP:55005-1-AP, 5ug; Detection:55005-1-AP 1:300) with MCF-7 cells lysate 3250ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human breast cancer using 55005-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55005-1-AP (SETD1B Antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary tumor using 55005-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 55005-1-AP (SETD1B Antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary tumor using 55005-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 55005-1-AP (SETD1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 55005-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55005-1-AP (SETD1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 55005-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55005-1-AP (SETD1B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 55005-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55005-1-AP (SETD1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary tumor using 55005-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 55005-1-AP (SETD1B Antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 55005-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55005-1-AP (SETD1B Antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 55005-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SETD1B antibody (55005-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: human brain tissue, MCF-7 cells, mouse liver tissue
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human breast cancer tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55005-1-AP targets SETD1B in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: SET domain containing 1B
• Calculated Molecular Weight: 209 kDa
• Observed Molecular Weight: 250-260 kDa, 450 kDa
• GenBank Accession Number: NM_015048
• Gene Symbol: SETD1B
• Gene ID (NCBI): 23067
• RRID: AB_10858389
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UPS6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SETD1B, also named as KMT2G. Set1B associates with a about 450 kDa complex that contains all five non-catalytic components of the Set1A complex, including CFP1, Rbbp5, Ash2, Wdr5, and Wdr82. The complex is a histone methyltransferase that produces trimethylated histone H3 at Lys4. Set1B is widely expressed, and localize to a largely non-overlapping set of euchromatic nuclear speckles, suggesting that Set1B bind to a unique set of target genes and thus make non-redundant contributions to the epigenetic control of chromatin structure and gene expression( PMID: 17355966). The antibody is specific to SETD1B and, in westernbloting experiments, two specific bands can be detected, about 250 kDa, 450 kDa respectively, in addition, a unknown band around 70 kDa. This antibody is a rabbit polyclonal antibody raised against a region of human SETD1B.

Publications

What published studies show

The anti-H3K4me3 antibody (Cat# 55005-1-AP) has 11 citations published in journals including EMBO Journal, Cell Reports, Life Sciences, Molecular Cancer Research, International Journal of Molecular Sciences, International Immunopharmacology, Biogerontology, BBA – Molecular and Cellular Research, Neuroscience, American Journal of Cancer Research, and Histochemistry and Cell Biology. Research fields span epigenetics/histone modification, oncology, neurology (ischemic stroke), immunology/inflammation, and reproductive aging.

Automatically generated from the 11 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	EMBO J; Histone methyltransferases MLL2 and SETD1A/B play distinct roles in H3K4me3 deposition during the transition from totipotency to pluripotency; Authors - Jingjing Zhang ; KD Validated
mouse	WB	Cell Rep; A program of successive gene expression in mouse one-cell embryos; Authors - Maki Asami
mouse	WB	Life Sci; SETD1B promotes brain cell ferroptosis in ischemic stroke mice via increasing H3K4me3 enrichment on the Tfrc promoter; Authors - Li Wang
human	ChIP	Mol Cancer Res; Downregulation of BASP1 promotes temozolomide resistance in gliomas via epigenetic activation of the FBXO32/NF-κB/MGMT axis; Authors - Xinyi Liao
mouse	WB,IHC,ChIP	Int Immunopharmacol; NF-κB p65 and SETDB1 expedite lipopolysaccharide-induced intestinal inflammation in mice by inducing IRF7/NLR-dependent macrophage M1 polarization; Authors - Li Chen
human	WB	Int J Mol Sci; (20S) Ginsenoside Rh2 Exerts Its Anti-Tumor Effect by Disrupting the HSP90A-Cdc37 System in Human Liver Cancer Cells.; Authors - Chen Chen