SETD3 Polyclonal antibody, PBS Only

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  WB analysis using 28692-1-AP (same clone as 28692-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 28692-1-AP (SETD3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28692-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 28692-1-AP (same clone as 28692-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 28692-1-AP (SETD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28692-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 28692-1-AP (same clone as 28692-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28692-1-AP (SETD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28692-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 28692-1-AP (same clone as 28692-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using SETD3 antibody (28692-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 28692-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 28692-1-AP (same clone as 28692-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using SETD3 antibody (28692-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 28692-1-PBS in a different storage buffer formulation.

SETD3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 28692-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

C14orf154, SET domain containing 3

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Product Information

28692-1-PBS targets SETD3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag30017

  Product name: Recombinant human SETD3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-120 aa of NM_032233

  Sequence: MGKKSRVKTQKSGTGATATVSPKEILNLTSELLQKCSSPAPGPGKEWEEYVQIRTLVEKIRKKQKGLSVTFDGKREDYFPDLMKWASENGASVEGFEMVNFKEEGFGLRATRDIKAEELF

• Full Name: SET domain containing 3
• Calculated Molecular Weight: 67 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: NM_032233
• Gene Symbol: SETD3
• Gene ID (NCBI): 84193
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86TU7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SETD3 (SET domain protein 3), a ubiquitously expressed protein, is epigenetically up-regulated in response to muscle loading and hypertrophy. The SETD3 mRNA is ubiquitously expressed at a similar basal level in most examined tissues, with the noticeable exception of the skeletal muscle, kidneys, and testes (PMID: 34685411). SETD3 deficient female mice have severely decreased litter sizes due to primary maternal dystocia that is refractory to ecbolic induction agents (PMID: 30626964).