|Positive WB detected in||A549 cells, HeLa cells, HepG2 cells, SKOV-3 cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11044-1-AP targets SFRS7 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||SFRS7 fusion protein Ag1526|
|Full Name||splicing factor, arginine/serine-rich 7, 35kDa|
|Calculated molecular weight||27 kDa|
|Observed molecular weight||35 kDa|
|GenBank accession number||BC000997|
|Gene ID (NCBI)||6432|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
SFRS7 is a member of the serine/arginine (SR) splicing factor family, which mediated the alternative splicing events target the resulting mRNAs for degradation by means of an RNA surveillance pathway called nonsense-mediated mRNA decay. It was found to repress the splicing of MAPT/Tau exon 10
Genomics Proteomics Bioinformatics
Specific Regulation of m6A by SRSF7 Promotes the Progression of Glioblastoma.
Nucleic Acids Res
Exonic splicing code and protein binding sites for calcium.
Nucleic Acids Res
Sequence-dependent recruitment of SRSF1 and SRSF7 to intronless lncRNA NKILA promotes nuclear export via the TREX/TAP pathway.
C9orf72 Dipeptide Repeats Impair the Assembly, Dynamics, and Function of Membrane-Less Organelles.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Jingwen (Verified Customer) (04-28-2021)
The staining signal showed up inside the nucleus.