SFRS7 Polyclonal antibody

• Featured Product
• KD/KO Validated

SFRS7 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 11044-1-AP

Synonyms

9G8, AAG3, SFRS7, Splicing factor 9G8, SRSF7

---

Validation Data Gallery

• 

  WB analysis using 11044-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11044-1-AP (SFRS7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 11044-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 11044-1-AP (SFRS7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11044-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11044-1-AP (SFRS7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 11044-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11044-1-AP (SFRS7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11044-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11044-1-AP (SFRS7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11044-1-AP

  Immunofluorescent analysis of HepG2 cells, using SFRS7 antibody 11044-1-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

---

Tested Applications

• Positive WB detected in: A549 cells, mouse testis tissue, rat testis tissue, HeLa cells, HepG2 cells, SKOV-3 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 4 publications below

Product Information

11044-1-AP targets SFRS7 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SFRS7 fusion protein Ag1526
• Full Name: splicing factor, arginine/serine-rich 7, 35kDa
• Calculated molecular weight: 27 kDa
• Observed molecular weight: 35 kDa
• GenBank accession number: BC000997
• Gene symbol: SFRS7
• Gene ID (NCBI): 6432
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SFRS7 is a member of the serine/arginine (SR) splicing factor family, which mediated the alternative splicing events target the resulting mRNAs for degradation by means of an RNA surveillance pathway called nonsense-mediated mRNA decay. It was found to repress the splicing of MAPT/Tau exon 10

Publications

Species	Application	Title
human	WB	Cell; C9orf72 Dipeptide Repeats Impair the Assembly, Dynamics, and Function of Membrane-Less Organelles.; Authors - Kyung-Ha Lee
human	WB	Nucleic Acids Res; Sequence-dependent recruitment of SRSF1 and SRSF7 to intronless lncRNA NKILA promotes nuclear export via the TREX/TAP pathway.; Authors - Misbah Khan ; KD Validated
human	WB	Nucleic Acids Res; Exonic splicing code and protein binding sites for calcium.; Authors - Reuben J Pengelly ; KD Validated
human	WB	Genomics Proteomics Bioinformatics; Specific Regulation of m6A by SRSF7 Promotes the Progression of Glioblastoma.; Authors - Yixian Cun ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Jingwen (Verified Customer) (04-28-2021)

The staining signal showed up inside the nucleus.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:150
• Cell Tissue Type: Human cancer cell line