SFRS7 Polyclonal antibody

SFRS7 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11044-1-AP

Synonyms

9G8, AAG3, SFRS7, Splicing factor 9G8, SRSF7



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11044-1-AP targets SFRS7 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SFRS7 fusion protein Ag1526
Full Name splicing factor, arginine/serine-rich 7, 35kDa
Calculated molecular weight 27 kDa
Observed molecular weight35 kDa
GenBank accession numberBC000997
Gene symbol SFRS7
Gene ID (NCBI) 6432
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SFRS7 is a member of the serine/arginine (SR) splicing factor family, which mediated the alternative splicing events target the resulting mRNAs for degradation by means of an RNA surveillance pathway called nonsense-mediated mRNA decay. It was found to repress the splicing of MAPT/Tau exon 10

Protocols

Product Specific Protocols
WB protocol for SFRS7 antibody 11044-1-APDownload protocol
IHC protocol for SFRS7 antibody 11044-1-APDownload protocol
IF protocol for SFRS7 antibody 11044-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nucleic Acids Res

Sequence-dependent recruitment of SRSF1 and SRSF7 to intronless lncRNA NKILA promotes nuclear export via the TREX/TAP pathway.

Authors - Misbah Khan
  • KD Validated
humanWB

Cell

C9orf72 Dipeptide Repeats Impair the Assembly, Dynamics, and Function of Membrane-Less Organelles.

Authors - Kyung-Ha Lee

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Jingwen (Verified Customer) (04-28-2021)

The staining signal showed up inside the nucleus.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:150
  • Cell Tissue Type: Human cancer cell line