SFRS8 Polyclonal antibody

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• SFRS8 Polyclonal antibody (24705-1-AP)

SFRS8 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 24705-1-AP Copied!

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

SFSWAP, Splicing factor, arginine/serine-rich 8, Splicing factor, suppressor of white-apricot homolog, SWAP

• 

  WB analysis of Jurkat using 24705-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24705-1-AP (SFRS8 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 24705-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24705-1-AP (SFRS8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of A549 using 24705-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using SFRS8 antibody (24705-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 24705-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SFRS8 antibody (24705-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells
• Positive IF/ICC detected in: A549 cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24705-1-AP targets SFRS8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19762

  Product name: Recombinant human SFRS8 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 600-951 aa of BC136678

  Sequence: LDDDSDDDEESKEGQESSSSAANTNPAVAPPCVVVEEKKPQLTQEELEAKQAKQKLEDRLAAAAREKLAQASKESKEKQLQAERKRKAALFLQTLKNPLPEAEAGKIEESPFSVEESSTTPCPLLTGGRPLPTLEVKPPDRPSSKSKDPPREEEKEKKKKKHKKRSRTRSRSPKYHSSSKSRSRSHSKAKHSLPSAYRTVRRSRSRSRSPRRRAHSPERRREERSVPTAYRVSRSPGASRKRTRSRSPHEKKKKRRSRSRTKSKARSQSVSPSKQAAPRPAAPAAHSAHSASVSPVESRGSSQERSRGVSQEKEAQISSAIVSSVQSKITQDLMAKVRAMLAASKNLQTSAS

• Full Name: splicing factor, arginine/serine-rich 8 (suppressor-of-white-apricot homolog, Drosophila)
• Calculated Molecular Weight: 951 aa, 105 kDa
• Observed Molecular Weight: 130-150 kDa
• GenBank Accession Number: BC136678
• Gene Symbol: SFRS8
• Gene ID (NCBI): 6433
• RRID: AB_2879682
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q12872
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SFRS8, also named as SFSWAP and SWAP, is splicing regulator. It regulates the splicing of fibronectin and CD45 genes. SFRS8 represses the splicing of MAPT/Tau exon 10. This antibody detects 130-150 kDa SFRS8 protein.

Publications

What published studies show

The antibody Anti-SFRS8 (Cat# 24705-1-AP) has 2 total citations. Citations appear in Clinical and Translational Medicine (IF 7.9) and Molecular Neurobiology (IF 5.5). Research fields include multiple myeloma malignancy (alternative splicing of CACYBP and exosome-based cellular communication) and neuroscience (memory regulation via RNA splicing of synaptic genes). Applications cited include WB and IHC in mouse and human samples.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse,human	WB,IHC	Clin Transl Med; Splicing factor arginine/serine-rich 8 promotes multiple myeloma malignancy and bone lesion through alternative splicing of CACYBP and exosome-based cellular communication.; Authors - Yuanjiao Zhang
	WB	Mol Neurobiol; SFRS8 Regulates Memory by Modulating RNA Splicing of Synaptic Genes; Authors - Zeng Ding