SFRS8 Polyclonal antibody

SFRS8 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 24705-1-AP

Synonyms

SFRS8, SWAP

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Validation Data Gallery

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  WB analysis of Jurkat using 24705-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24705-1-AP (SFRS8 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24705-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24705-1-AP (SFRS8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 24705-1-AP

  Immunofluorescent analysis of HeLa cells using 24705-1-AP (SFRS8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 24705-1-AP is SFRS8 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SFRS8 fusion protein Ag19762
• Full Name: splicing factor, arginine/serine-rich 8 (suppressor-of-white-apricot homolog, Drosophila)
• Calculated Molecular Weight: 951 aa, 105 kDa
• Observed Molecular Weight: 130-150 kDa
• GenBank Accession Number: BC136678
• Gene Symbol: SFRS8
• Gene ID (NCBI): 6433
• RRID: AB_2879682
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SFRS8, also named as SFSWAP and SWAP, is splicing regulator. It regulates the splicing of fibronectin and CD45 genes. SFRS8 represses the splicing of MAPT/Tau exon 10. This antibody detects 130-150 kDa SFRS8 protein.

Publications

Species	Application	Title
human,mouse	WB,IHC	Clin Transl Med; Splicing factor arginine/serine-rich 8 promotes multiple myeloma malignancy and bone lesion through alternative splicing of CACYBP and exosome-based cellular communication.; Authors - Yuanjiao Zhang