SFT2D2 Polyclonal antibody

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  WB analysis using 32914-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32914-1-AP (SFT2D2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 32914-1-AP

  IP result of anti-SFT2D2 (IP:32914-1-AP, 4ug; Detection:32914-1-AP 1:500) with HeLa cells lysate 960 ug.

SFT2D2 Polyclonal Antibody for WB, IP, ELISA

Cat No. 32914-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

SFT2 domain-containing protein 2, UNQ512/PRO1027, Vesicle transport protein SFT2B

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Tested Applications

• Positive WB detected in: A549 cells, Calu-1 cells, HeLa cells, MCF-7 cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32914-1-AP targets SFT2D2 in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag36996

  Product name: Recombinant human SFT2D2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-63 aa of BC068098

  Sequence: MDKLKKVLSGQDTEDRSGLSEVVEASSLSWSTRIKGFIACFAIGILCSLLGTVLLWVPRKGLH

• Full Name: SFT2 domain containing 2
• Calculated Molecular Weight: 160 aa, 18 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC068098
• Gene Symbol: SFT2D2
• Gene ID (NCBI): 375035
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: O95562
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SFT2D2 is part of the SFT2 protein family, which is involved in managing membrane traffic inside cells. Its specific molecular role is linked to the Golgi apparatus, a central hub for processing and sorting proteins.