SGLT1 Polyclonal antibody, PBS Only

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  WB analysis using 30861-1-AP (same clone as 30861-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30861-1-AP (SGLT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30861-1-PBS in a different storage buffer formulation.

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  WB analysis using 30861-1-AP (same clone as 30861-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30861-1-AP (SGLT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30861-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse kidney using 30861-1-AP (same clone as 30861-1-PBS)

  IP result of anti-SGLT1 (IP:30861-1-AP, 4ug; Detection:30861-1-AP 1:400) with mouse kidney tissue lysate 2160 ug. This data was developed using the same antibody clone with 30861-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 30861-1-AP (same clone as 30861-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 30861-1-AP (SGLT1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30861-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 30861-1-AP (same clone as 30861-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 30861-1-AP (SGLT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30861-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 30861-1-AP (same clone as 30861-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 30861-1-AP (SGLT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30861-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse small intestine using 30861-1-AP (same clone as 30861-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using SGLT1 antibody (30861-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30861-1-PBS in a different storage buffer formulation.

SGLT1 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA

Cat No. 30861-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Synonyms

SLC5A1, High affinity sodium-glucose cotransporter

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Product Information

30861-1-PBS targets SGLT1 in WB, IHC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SGLT1 fusion protein Ag29338
• Full Name: solute carrier family 5 (sodium/glucose cotransporter), member 1
• Calculated Molecular Weight: 73 kDa
• Observed Molecular Weight: 73 kDa
• GenBank Accession Number: NM_000343
• Gene Symbol: SGLT1
• Gene ID (NCBI): 6523
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P13866
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

SLC5A1, also known as SGLT1, is a sodium-dependent glucose transporter (SGLT) family member. SGLT1 couples sugar transport to Na+ gradients across the intestinal brush border (PMID: 8563765). SGLT1 mediates active glucose and galactose absorption in the intestine and renal glucose scavenging. Mutations in SGLT1 cause glucose-galactose malabsorption (GGM) syndrome (PMID: 34880492). SGLT1 is expressed in intestine and endometrial cells (PMID: 2490366, 28974690).