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  • KD/KO Validated

SHC Polyclonal antibody

SHC Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 10054-1-AP

Synonyms

SH2 domain protein C1, SHC, SHC transforming protein 1, SHC transforming protein 3, SHC transforming protein A, SHC1, SHCA



Tested Applications

Positive WB detected inC6 cells, A431 cells, mouse skin tissue, HeLa cells, U2OS cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10054-1-AP targets SHC in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SHC fusion protein Ag0103
Full Name SHC (Src homology 2 domain containing) transforming protein 1
Calculated molecular weight 63 kDa
Observed molecular weight 66 kDa
GenBank accession numberNM_183001
Gene symbol SHC1
Gene ID (NCBI) 6464
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for SHC antibody 10054-1-APDownload protocol
IHC protocol for SHC antibody 10054-1-APDownload protocol
IF protocol for SHC antibody 10054-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
sheepIF

Mol Reprod Dev

p66Shc is associated with hydrogen peroxide-induced oxidative stress in preimplantation sheep embryos.

Authors - Tong Zhang
mouseIF

Oxid Med Cell Longev

Protocatechuic Acid-Mediated miR-219a-5p Activation Inhibits the p66shc Oxidant Pathway to Alleviate Alcoholic Liver Injury.

Authors - Rong Fu
  • KD Validated
mouseWB

Aging (Albany NY)

RNA-seq analysis of the key long noncoding RNAs and mRNAs related to cognitive impairment after cardiac arrest and cardiopulmonary resuscitation.

Authors - Chan Chen
humanWB

Eur Rev Med Pharmacol Sci

Oxidative stress induces B lymphocyte DNA damage and apoptosis by upregulating p66shc.

Authors - J-Y Lang
humanWB

Clinics (Sao Paulo)

Ketamine ameliorates hypoxia-induced endothelial injury in human umbilical vein endothelial cells.

Authors - Xiaohui Zhou
mouseWB

Aging (Albany NY)

Targeting of miR-96-5p by catalpol ameliorates oxidative stress and hepatic steatosis in LDLr-/- mice via p66shc/cytochrome C cascade.

Authors - Yukun Zhang