SHC Polyclonal antibody

• Featured Product
• KD/KO Validated

SHC Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 10054-1-AP

Synonyms

SH2 domain protein C1, SHC, SHC transforming protein 1, SHC transforming protein 3, SHC transforming protein A, SHC1, SHCA

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Validation Data Gallery

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  WB analysis using 10054-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10054-1-AP (SHC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10054-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10054-1-AP (SHC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10054-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10054-1-AP (SHC antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skin using 10054-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 10054-1-AP (SHC antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 10054-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10054-1-AP (SHC antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 10054-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10054-1-AP (SHC antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 10054-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 10054-1-AP (SHC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: C6 cells, A431 cells, mouse skin tissue, HUVEC cells, HeLa cells, U2OS cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

10054-1-AP targets SHC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SHC fusion protein Ag0103
• Full Name: SHC (Src homology 2 domain containing) transforming protein 1
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: NM_183001
• Gene Symbol: SHC1
• Gene ID (NCBI): 6464
• RRID: AB_513584
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The proto-oncogene SHC family consists of SHCA, SHCB, SHCC, and SHCD4 gene members, which can encode 7 kinds of cohesive molecules. SHC family is widely expressed and functional. The SHC1 gene is located in zone 1, region 2 of human chromosome 1. SHC1 is believed to be involved in inhibiting neuronal apoptosis and regulating the receptor tyrosine kinase pathway. SHC1 sensitizes cancer cells to the 8-Cl-cAMP treatment.

Publications

Species	Application	Title
human	WB	Clin Transl Med; Aptamer-SH2 superbinder-based targeted therapy for pancreatic ductal adenocarcinoma.; Authors - An-Dong Liu
mouse	IF	Oxid Med Cell Longev; Protocatechuic Acid-Mediated miR-219a-5p Activation Inhibits the p66shc Oxidant Pathway to Alleviate Alcoholic Liver Injury.; Authors - Rong Fu ; KD Validated
mouse	WB	Aging (Albany NY); RNA-seq analysis of the key long noncoding RNAs and mRNAs related to cognitive impairment after cardiac arrest and cardiopulmonary resuscitation.; Authors - Chan Chen
mouse	WB	Aging (Albany NY); Targeting of miR-96-5p by catalpol ameliorates oxidative stress and hepatic steatosis in LDLr-/- mice via p66shc/cytochrome C cascade.; Authors - Yukun Zhang
rat	WB	J Ethnopharmacol; Dahuang Zhechong pill attenuates hepatic sinusoidal capillarization in liver cirrhosis and hepatocellular carcinoma rat model via MK/integrin signaling pathway; Authors - Chuankui Fu
mouse	WB	Stem Cell Res Ther; Oncostatin M receptor regulates osteoblast differentiation via extracellular signal-regulated kinase/autophagy signaling.; Authors - Jie Zhou