|Positive WB detected in||C6 cells, A431 cells, mouse skin tissue, HeLa cells, U2OS cells|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10054-1-AP targets SHC in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, sheep|
|Host / Isotype||Rabbit / IgG|
|Immunogen||SHC fusion protein Ag0103|
|Full Name||SHC (Src homology 2 domain containing) transforming protein 1|
|Calculated molecular weight||63 kDa|
|Observed molecular weight||66 kDa|
|GenBank accession number||NM_183001|
|Gene ID (NCBI)||6464|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Mol Reprod Dev
p66Shc is associated with hydrogen peroxide-induced oxidative stress in preimplantation sheep embryos.
Oxid Med Cell Longev
Protocatechuic Acid-Mediated miR-219a-5p Activation Inhibits the p66shc Oxidant Pathway to Alleviate Alcoholic Liver Injury.
Aging (Albany NY)
RNA-seq analysis of the key long noncoding RNAs and mRNAs related to cognitive impairment after cardiac arrest and cardiopulmonary resuscitation.
Eur Rev Med Pharmacol Sci
Oxidative stress induces B lymphocyte DNA damage and apoptosis by upregulating p66shc.
Clinics (Sao Paulo)
Ketamine ameliorates hypoxia-induced endothelial injury in human umbilical vein endothelial cells.
Aging (Albany NY)
Targeting of miR-96-5p by catalpol ameliorates oxidative stress and hepatic steatosis in LDLr-/- mice via p66shc/cytochrome C cascade.