SHPK Polyclonal antibody, PBS Only

• 

  WB analysis using 12441-1-AP (same clone as 12441-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12441-1-AP (SHPK antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12441-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 12441-1-AP (same clone as 12441-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12441-1-AP (SHPK antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12441-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 12441-1-AP (same clone as 12441-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12441-1-AP (SHPK antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12441-1-PBS in a different storage buffer formulation.

SHPK Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 12441-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

12441-1-PBS targets SHPK in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3150

  Product name: Recombinant human SHPK protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 131-478 aa of BC020543

  Sequence: RCSSEFLASLPQPKSHLSVATGFGCATIFWLLKYRPEFLKSYDAAGTIHDYVVAMLCGLPRPLMSDQNAASWGYFNTQSQSWNVETLRSSGFPVHLLPDIAEPGSVAGRTSHMWFEIPKGTQVGVALGDLQASVYSCMAQRTDAVLNISTSVQLAASMPSGFQPAQTPDPTAPVAYFPYFNRTYLGVAASLNGGNVLATFVHMLVQWMADLGLEVEESTVYSRMIQAAVQQRDTHLTITPTVLGERHLPDQLASVTRISSSDLSLGHVTRALCRGIVQNLHSMLPIQQLQEWGVERVMGSGSALSRNDVLKQEVQRAFPLPMSFGQDVDAAVGAALVMLRRHLNQKES

• Full Name: sedoheptulokinase
• Calculated Molecular Weight: 478 aa, 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC020543
• Gene Symbol: SHPK
• Gene ID (NCBI): 23729
• RRID: AB_2188566
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UHJ6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

SHPK, also named CARKL, is a carbohydrate kinase involved in the phosphorylation of sugars as they enter a cell, inhibiting return across the cell membrane (PubMed: 10673275). SHPK is a sedoheptulose kinase of the pentose phosphate pathway. It acts as a modulator of macrophage activation through control of glucose metabolism (PMID: 22682222).