SIRP Alpha/CD172a Polyclonal antibody

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  WB analysis using 14482-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14482-1-AP (SIRP Alpha/CD172a antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 14482-1-AP

  WB result of SIRP Alpha/CD172a antibody (14482-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SIRP Alpha/CD172a transfected U-937 cells.

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  WB analysis using 14482-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14482-1-AP (SIRP Alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14482-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14482-1-AP (SIRP Alpha/CD172a antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 14482-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 14482-1-AP (SIRP alpha antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 14482-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 14482-1-AP (SIRP Alpha/CD172a antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of THP-1 using 14482-1-AP

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using SIRP Alpha/CD172a antibody (14482-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

SIRP Alpha/CD172a Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 14482-1-AP

Publications(4)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

SIRP Alpha, SIRPA, BIT, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A

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Tested Applications

• Positive WB detected in: THP-1 cells, HL-60 cells, PC-12 cells, U-937 cells, mouse cerebellum tissue, rat cerebellum tissue
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

14482-1-AP targets SIRP Alpha/CD172a in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5865

  Product name: Recombinant human SIRPA protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 158-508 aa of BC033092

  Sequence: ARATPQHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPVGESVSYSIHSTAKVVLTREDVHSQVICEVAHVTLQGDPLRGTANLSETIRVPPTLEVTQQPVRAENQVNVTCQVRKFYPQRLQLTWLENGNVSRTETASTVTENKDGTYNWMSWLLVNVSAHRDDVKLTCQVEHDGQPAVSKSHDLKVSAHPKEQGSNTAAENTGSNERNIYIVVGVVCTLLVALLMAALYLVRIRQKKAQGSTSSTRLHEPEKNAREITQVQSLDTNDITYADLNLPKGKKPAPQAAEPNNHTEYASIQTSPQPASEDTLTYADLDMVHLNRTPKQPAPKPEPSFSEYASVQVPRK

• Full Name: signal-regulatory protein alpha
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 75-85 kDa
• GenBank Accession Number: BC033092
• Gene Symbol: SIRP Alpha
• Gene ID (NCBI): 140885
• RRID: AB_2188056
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P78324
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954). This antibody detects a 75-85 kDa band.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Cell Prolif; Mitochondrial DNA mediates immunoparalysis of dendritic cells in sepsis via STING signalling; Authors - Qing Tu
mouse	WB	Acta Biochim Biophys Sin (Shanghai); Clostridium butyricum regulates visceral hypersensitivity of irritable bowel syndrome by inhibiting colonic mucous low grade inflammation through its action on NLRP6.; Authors - Kejia Zhao
human	WB	J Obstet Gynaecol Res; Eutopic endometrium from patients with endometriosis modulates the expression of CD36 and SIRP-α in peritoneal macrophages.; Authors - Qi Xie
human	WB	Biomedicines; Exosomes from the Uterine Cavity Mediate Immune Dysregulation via Inhibiting the JNK Signal Pathway in Endometriosis; Authors - Ying Jiang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Manuel (Verified Customer) (03-19-2025)

Works well.

• Applications: Western Blot, Immunoprecipitation
• Cell Tissue Type: THP-1

FH

Rebecca (Verified Customer) (05-27-2024)

Transference at 4ºC and 90V, for 2h. Antibody incubation ON at 4ºC. Multiple bands observed. A bigger dilution of the antibody is probably needed.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: hiPSC-CM

SIRP Alpha/CD172a Antibody Western Blot validation (1:1000 dilution) in hiPSC-CM (Cat no:14482-1-AP)