SIRP Alpha/CD172a Polyclonal antibody

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SIRP Alpha/CD172a Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 14482-1-AP

Publications(6)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

SIRP Alpha, SIRPA, BIT, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A

• 

  WB analysis using 14482-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14482-1-AP (SIRP Alpha/CD172a antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 14482-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14482-1-AP (SIRP Alpha/CD172a antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 14482-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14482-1-AP (SIRP Alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of U-937 using 14482-1-AP

  WB result of SIRP Alpha/CD172a antibody (14482-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SIRP Alpha/CD172a transfected U-937 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 14482-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14482-1-AP (SIRP Alpha/CD172a antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of PC-12 using 14482-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 14482-1-AP (SIRP alpha antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of THP-1 using 14482-1-AP

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using SIRP Alpha/CD172a antibody (14482-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: THP-1 cells, HL-60 cells, mouse brain tissue, PC-12 cells, U-937 cells, rat brain tissue, mouse cerebellum tissue
• Positive IF/ICC detected in: THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14482-1-AP targets SIRP Alpha/CD172a in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5865

  Product name: Recombinant human SIRPA protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 158-508 aa of BC033092

  Sequence: ARATPQHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPVGESVSYSIHSTAKVVLTREDVHSQVICEVAHVTLQGDPLRGTANLSETIRVPPTLEVTQQPVRAENQVNVTCQVRKFYPQRLQLTWLENGNVSRTETASTVTENKDGTYNWMSWLLVNVSAHRDDVKLTCQVEHDGQPAVSKSHDLKVSAHPKEQGSNTAAENTGSNERNIYIVVGVVCTLLVALLMAALYLVRIRQKKAQGSTSSTRLHEPEKNAREITQVQSLDTNDITYADLNLPKGKKPAPQAAEPNNHTEYASIQTSPQPASEDTLTYADLDMVHLNRTPKQPAPKPEPSFSEYASVQVPRK

• Full Name: signal-regulatory protein alpha
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 75-85 kDa
• GenBank Accession Number: BC033092
• Gene Symbol: SIRP Alpha
• Gene ID (NCBI): 140885
• RRID: AB_2188056
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P78324
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954). This antibody detects a 75-85 kDa band.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Cell Prolif; Mitochondrial DNA mediates immunoparalysis of dendritic cells in sepsis via STING signalling; Authors - Qing Tu
mouse	WB	Acta Biochim Biophys Sin (Shanghai); Clostridium butyricum regulates visceral hypersensitivity of irritable bowel syndrome by inhibiting colonic mucous low grade inflammation through its action on NLRP6.; Authors - Kejia Zhao
	WB	Alzheimers Dement; Gut-derived bacterial vesicles carrying lipopolysaccharide promote microglia-mediated synaptic pruning; Authors - Xiaoduo Zhao
mouse	WB	ACS Appl Mater Interfaces; Genetically Engineered Membrane-Mimetic Liposome-Wrapped Violet Phosphorus Nanoplatform for Targeted Synergistic Ferroptosis/Photothermal/Immunotherapy of Hepatocellular Carcinoma.; Authors - Shuo Li
human	WB	Biomedicines; Exosomes from the Uterine Cavity Mediate Immune Dysregulation via Inhibiting the JNK Signal Pathway in Endometriosis; Authors - Ying Jiang
human	WB	J Obstet Gynaecol Res; Eutopic endometrium from patients with endometriosis modulates the expression of CD36 and SIRP-α in peritoneal macrophages.; Authors - Qi Xie

Reviews

4.5 out of 5

2 verified customer reviews

What customers say

Automatically generated from the verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Manuel (Verified Customer) (03-19-2025)

Works well.

• Applications: Western Blot, Immunoprecipitation
• Cell Tissue Type: THP-1

Rebecca (Verified Customer) (05-27-2024)

Transference at 4ºC and 90V, for 2h. Antibody incubation ON at 4ºC. Multiple bands observed. A bigger dilution of the antibody is probably needed.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: hiPSC-CM

SIRP Alpha/CD172a Antibody Western Blot validation (1:1000 dilution) in hiPSC-CM (Cat no:14482-1-AP)