Tested Applications
| Positive WB detected in | THP-1 cells, HL-60 cells, mouse brain tissue, PC-12 cells, U-937 cells, rat brain tissue, mouse cerebellum tissue |
| Positive IF/ICC detected in | THP-1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14482-1-AP targets SIRP Alpha/CD172a in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5865 Product name: Recombinant human SIRPA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 158-508 aa of BC033092 Sequence: ARATPQHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPVGESVSYSIHSTAKVVLTREDVHSQVICEVAHVTLQGDPLRGTANLSETIRVPPTLEVTQQPVRAENQVNVTCQVRKFYPQRLQLTWLENGNVSRTETASTVTENKDGTYNWMSWLLVNVSAHRDDVKLTCQVEHDGQPAVSKSHDLKVSAHPKEQGSNTAAENTGSNERNIYIVVGVVCTLLVALLMAALYLVRIRQKKAQGSTSSTRLHEPEKNAREITQVQSLDTNDITYADLNLPKGKKPAPQAAEPNNHTEYASIQTSPQPASEDTLTYADLDMVHLNRTPKQPAPKPEPSFSEYASVQVPRK Predict reactive species |
| Full Name | signal-regulatory protein alpha |
| Calculated Molecular Weight | 55 kDa |
| Observed Molecular Weight | 75-85 kDa |
| GenBank Accession Number | BC033092 |
| Gene Symbol | SIRP Alpha |
| Gene ID (NCBI) | 140885 |
| RRID | AB_2188056 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P78324 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954). This antibody detects a 75-85 kDa band.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SIRP Alpha/CD172a antibody 14482-1-AP | Download protocol |
| WB protocol for SIRP Alpha/CD172a antibody 14482-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SIRP Alpha/CD172a Polyclonal antibody (Cat# 14482-1-AP) has 6 total citations. These appear in Alzheimer's & Dementia, ACS Applied Materials & Interfaces, Cell Proliferation, Biomedicines, Acta Biochimica et Biophysica Sinica, and Journal of Obstetrics and Gynaecology Research. The associated research fields include neurodegeneration and synaptic pruning, hepatocellular carcinoma immunotherapy, sepsis-related immune dysfunction, endometriosis-associated immune dysregulation, and intestinal inflammation. All cited applications are Western blot.
| Species | Application | Title |
|---|---|---|
Alzheimers Dement Gut-derived bacterial vesicles carrying lipopolysaccharide promote microglia-mediated synaptic pruning | ||
ACS Appl Mater Interfaces Genetically Engineered Membrane-Mimetic Liposome-Wrapped Violet Phosphorus Nanoplatform for Targeted Synergistic Ferroptosis/Photothermal/Immunotherapy of Hepatocellular Carcinoma. | ||
Cell Prolif Mitochondrial DNA mediates immunoparalysis of dendritic cells in sepsis via STING signalling | ||
Acta Biochim Biophys Sin (Shanghai) Clostridium butyricum regulates visceral hypersensitivity of irritable bowel syndrome by inhibiting colonic mucous low grade inflammation through its action on NLRP6. | ||
Biomedicines Exosomes from the Uterine Cavity Mediate Immune Dysregulation via Inhibiting the JNK Signal Pathway in Endometriosis | ||
J Obstet Gynaecol Res Eutopic endometrium from patients with endometriosis modulates the expression of CD36 and SIRP-α in peritoneal macrophages. |
Reviews
What customers say
Both reviewers used the antibody for Western Blot. One user (5/5) reported it "works well" for WB and IP on THP-1 cells. The other (4/5) tested it on hiPSC-derived cardiomyocytes at 1:1000 dilution with overnight incubation at 4°C and observed multiple bands, suggesting a higher dilution may be needed to improve specificity. Overall, the antibody performs reliably but may require optimization of dilution depending on the sample type.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Manuel (Verified Customer) (03-19-2025) | Works well.
|
Rebecca (Verified Customer) (05-27-2024) | Transference at 4ºC and 90V, for 2h. Antibody incubation ON at 4ºC. Multiple bands observed. A bigger dilution of the antibody is probably needed.
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