• Featured Product
  • KD/KO Validated

OCTN2 Polyclonal antibody

OCTN2 Polyclonal Antibody for FC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, FC,ELISA

Conjugate

Unconjugated

Cat no : 16331-1-AP

Synonyms

CDSP, OCTN2, OCTN2VT, SLC22A5



Tested Applications

Positive WB detected inmouse small intestine tissue, mouse skeletal muscle tissue, human placenta tissue, mouse pancreas tissue, mouse testis tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

16331-1-AP targets OCTN2 in WB, IP, IHC, FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OCTN2 fusion protein Ag9450
Full Name solute carrier family 22 (organic cation/carnitine transporter), member 5
Calculated molecular weight 557 aa, 63 kDa
Observed molecular weight 70-80 kDa
GenBank accession numberBC012325
Gene symbol SLC22A5
Gene ID (NCBI) 6584
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

OCTN2 (Organic Cation/Carnitine Transporter 2), encoded by SLC22A5, is a ubiquitously expressed organic anion/cation transporter. OCTN2 plays a key role in absorption, tissue distribution and renal reabsorption of L-carnitine which is an essential cofactor in fat metabolism. Defects in the OCTN2 are responsible for primary carnitine deficiency. OCTN2 also transports some important respiratory medicines (such as tiotropium), and due to its expression in the lung, may influence the disposition and absorption of these medicines in the lung.

Protocols

Product Specific Protocols
WB protocol for OCTN2 antibody 16331-1-APDownload protocol
IHC protocol for OCTN2 antibody 16331-1-APDownload protocol
IP protocol for OCTN2 antibody 16331-1-APDownload protocol
FC protocol for OCTN2 antibody 16331-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Biomed Pharmacother

The flavonoid-rich fraction from rhizomes of Smilax glabra Roxb. ameliorates renal oxidative stress and inflammation in uric acid nephropathy rats through promoting uric acid excretion.

Authors - Siwei Wang
humanWB

J Neurochem

Identification of drug transporters contributing to oxaliplatin-induced peripheral neuropathy.

Authors - S Fujita
  • KD Validated
mouseWB

Cell Metab

Global Analysis of Plasma Lipids Identifies Liver-Derived Acylcarnitines as a Fuel Source for Brown Fat Thermogenesis.

Authors - Judith Simcox