SLC22A9 Polyclonal antibody

SLC22A9 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 27998-1-AP

Synonyms

FLJ23666, HOAT4, OAT4, OAT7, Organic anion transporter 4, SLC22A9, ust3, UST3H

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Validation Data Gallery

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  WB analysis of mouse liver using 27998-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 27998-1-AP (SLC22A9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 27998-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27998-1-AP (SLC22A9 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 27998-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27998-1-AP (SLC22A9 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 27998-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27998-1-AP (SLC22A9 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 27998-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 27998-1-AP (SLC22A9 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse liver tissue
• Positive IHC detected in: human liver cancer tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

27998-1-AP targets SLC22A9 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC22A9 fusion protein Ag27573
• Full Name: solute carrier family 22 (organic anion transporter), member 9
• Calculated Molecular Weight: 553 aa, 62 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC022379
• Gene Symbol: SLC22A9
• Gene ID (NCBI): 114571
• RRID: AB_2881034
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Drug Dev Res; The mechanism of Arhalofenate in alleviating hyperuricemia-Activating PPARγ thereby reducing caspase-1 activity.; Authors - Guihong Wang
mouse	WB,IHC	Behav Brain Res; Disorders in the gut and liver are involved in depression contagion between isosexual post-stroke depression mice and the healthy cohabitors; Authors - Hui Wang