ANT1/2 Polyclonal antibody

ANT1/2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 15997-1-AP

Synonyms

AAC1, ADP,ATP carrier protein 1, ADP/ATP translocase 1, ANT, ANT 1, ANT1/2, PEO2, PEO3, SLC25A4, T1

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Validation Data Gallery

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  WB analysis of human heart using 15997-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15997-1-AP (ANT1/2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 15997-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 15997-1-AP (ANT1/2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 15997-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15997-1-AP (ANT1/2antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15997-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15997-1-AP (ANT1/2antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 15997-1-AP

  IP result of anti-ANT1/2 (IP:15997-1-AP, 4ug; Detection:15997-1-AP 1:500) with mouse heart tissue lysate 4000ug.

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Tested Applications

• Positive WB detected in: human heart tissue, MCF-7 cells, mouse skeletal muscle tissue, mouse brain tissue
• Positive IP detected in: mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• CoIP: See 1 publications below

Product Information

15997-1-AP targets ANT1/2 in WB, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ANT1/2 fusion protein Ag7787
• Full Name: solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 4
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC008664
• Gene Symbol: SLC25A4
• Gene ID (NCBI): 291
• RRID: AB_2190199
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Adenine nucleotide translocators (ANTs) are mitochondrial carrier proteins that exchange ATP and ADP between the cytoplasm and the mitochondrial matrix. Four isoforms of ANT were identified: ANT1 (encoded by SLC25A4) is highly expressed in different tissues; ANT2 (encoded by SLC25A5) is predominantly expressed in proliferating, regenerating, or undifferentiated cells; ANT3 is ubiquitously expressed; ANT4 is mainly expressed in testis and germ cells. This antibody recognizes both of ANT1 and ANT2.

Publications

Species	Application	Title
pig	WB	J Biol Chem; A new circular RNA-encoded protein BIRC6-236aa inhibits transmissible gastroenteritis virus (TGEV)-induced mitochondrial dysfunction.; Authors - Xiaomin Zhao
mouse	WB	Neurochem Int; Curcumin prevents mitochondrial dysfunction in the brain of the senescence-accelerated mouse-prone 8.; Authors - Eckert Gunter P GP
human	WB	Dev Cell; Deregulation of ER-mitochondria contact formation and mitochondrial calcium homeostasis mediated by VDAC in fragile X syndrome; Authors - Ji Geng
human	WB,CoIP	iScience; MRPL12-ANT3 interaction involves in acute kidney injury via regulating MPTP of tubular epithelial cells; Authors - Xingzhao Ji
mouse	WB	Int J Biol Macromol; Mfn2R364W, Mfn2G176S, and Mfn2H165R mutations drive Charcot-Marie-Tooth type 2A disease by inducing apoptosis and mitochondrial oxidative phosphorylation damage; Authors - Yuanzhu Zhang