SLC35A3 Polyclonal antibody

SLC35A3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26874-1-AP

Synonyms

Golgi UDP GlcNAc transporter, NGT, SLC35A3

---

Validation Data Gallery

• 

  WB analysis using 26874-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26874-1-AP (SLC35A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 26874-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 26874-1-AP (SLC35A3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, LNCaP cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26874-1-AP targets SLC35A3 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLC35A3 fusion protein Ag25468
• Full Name: solute carrier family 35 (UDP-N-acetylglucosamine (UDP-GlcNAc) transporter), member A3
• Calculated Molecular Weight: 325 aa, 36 kDa
• Observed Molecular Weight: 32-36 and 41 kDa
• GenBank Accession Number: BC005136
• Gene Symbol: SLC35A3
• Gene ID (NCBI): 23443
• RRID: AB_2880665
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC35A3, also named as UDP-N-acetylglucosamine transporter(NGT), is located to the Golgi apparatus. SLC35A3 can form heterologous complexes with SLC35A2(UGT) and Mgats in the Golgi membrane which may promote biosynthesis of complex N-glycans. It has been reported that the mutation of SLC35A3 is associsted with skeletal dysplasia and epilepsies which could result from impaired glycosylation of proteins. SLC35A3 has some isoforms with 32-36 kDa and 41 kDa.(PMID: 25944901, 28777481  , 16344554, 23583405)