|Positive WB detected in||HepG2 cells|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||human spleen tissue, human stomach cancer tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 18566-1-AP is band 3/ AE1 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, Plasmodium falciparum, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||band 3/ AE1 fusion protein Ag13076|
|Full Name||solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group)|
|Calculated molecular weight||911 aa, 102 kDa|
|Observed molecular weight||95 kDa, 50 kDa|
|GenBank accession number||BC101570|
|Gene ID (NCBI)||6521|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Anion exchanger 1 (AE1), also known as band 3, is a 95-100 kDa transmembrane glycoprotein which is abundantly expressed in erythrocyte plasma membrane and mediates the electroneutral exchange of Cl- and HCO3-. It contains a 52-55 kDa C-terminal membrane crossing domain and a 43 kDa N-terminal cytoplasmic domain. Degradation of band 3 protein occurred during erythrocyte aging. The oxidative stress can also activate the proteolysis of band 3 through caspases. This antibody is expected to recognize the intact band 3 (95-100 kDa) and various degradation products (38-60 kDa). (PMID: 22890269, 29240292, 28130418)
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