band 3/ AE1 Polyclonal antibody

band 3/ AE1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18566-1-AP

Synonyms

AE 1, AE1, Anion exchange protein 1, Anion exchanger 1, band 3, Band 3 anion transport protein, SLC4A1



Tested Applications

Positive WB detected inHepG2 cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman spleen tissue, human stomach cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 18566-1-AP is band 3/ AE1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, Plasmodium falciparum, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen band 3/ AE1 fusion protein Ag13076
Full Name solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group)
Calculated molecular weight 911 aa, 102 kDa
Observed molecular weight 95 kDa, 50 kDa
GenBank accession numberBC101570
Gene symbol SLC4A1
Gene ID (NCBI) 6521
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Anion exchanger 1 (AE1), also known as band 3, is a 95-100 kDa transmembrane glycoprotein which is abundantly expressed in erythrocyte plasma membrane and mediates the electroneutral exchange of Cl- and HCO3-. It contains a 52-55 kDa C-terminal membrane crossing domain and a 43 kDa N-terminal cytoplasmic domain. Degradation of band 3 protein occurred during erythrocyte aging. The oxidative stress can also activate the proteolysis of band 3 through caspases. This antibody is expected to recognize the intact band 3 (95-100 kDa) and various degradation products (38-60 kDa). (PMID: 22890269, 29240292, 28130418)

Protocols

Product Specific Protocols
WB protocol for band 3/ AE1 antibody 18566-1-APDownload protocol
IHC protocol for band 3/ AE1 antibody 18566-1-APDownload protocol
IP protocol for band 3/ AE1 antibody 18566-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
Plasmodium falciparumWB

Malar J

The malaria parasite Plasmodium falciparum in red blood cells selectively takes up serum proteins that affect host pathogenicity.

Authors - Takahiro Tougan
ratIHC

Front Neurosci

Influx mechanisms in the embryonic and adult rat choroid plexus: a transcriptome study.

Authors - Norman R Saunders
ratIHC

PLoS One

Mechanisms that determine the internal environment of the developing brain: a transcriptomic, functional and ultrastructural approach.

Authors - Liddelow Shane A SA
humanIF

Stem Cells Int

Pyridoxal-5'-Phosphate Promotes Immunomodulatory Function of Adipose-Derived Mesenchymal Stem Cells through Indoleamine 2,3-Dioxygenase-1 and TLR4/NF-κB Pathway.

Authors - Cong Li
mouseWB

Sci Adv

Red blood cell-derived nanoerythrosome for antigen delivery with enhanced cancer immunotherapy.

Authors - Xiao Han
mouseWB

Blood

Ferroportin deficiency in erythroid cells causes serum iron deficiency and promotes hemolysis due to oxidative stress.

Authors - De-Liang Zhang