Serotonin transporter Polyclonal antibody

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  WB analysis using 19559-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 19559-1-AP (Serotonin transporter antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 19559-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19559-1-AP (Serotonin transporter antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse cerebellum using 19559-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using Serotonin transporter antibody (19559-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

Serotonin transporter Polyclonal Antibody for WB, IF-P, ELISA

Cat No. 19559-1-AP

Publications(14)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

SLC6A4, 5 HTT, 5 HTTLPR, 5HTT, hSERT

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, mouse brain tissue, rat brain tissue
• Positive IF-P detected in: mouse cerebellum tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 12 publications below
• IHC: See 4 publications below

Product Information

19559-1-AP targets Serotonin transporter in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: solute carrier family 6 (neurotransmitter transporter, serotonin), member 4
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 70 kDa and 140 kDa
• GenBank Accession Number: NM_001045
• Gene Symbol: Serotonin transporter
• Gene ID (NCBI): 6532
• RRID: AB_2878590
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P31645
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Serotonin transporter (SERT; SLC6A4) belongs to the sodium- and chloride-dependent monoamine transporter family. SERT is a membrane transporter that terminates the neurotransmission of serotonin, a monoaminergic neurotransmitter, through its reuptake. The SERT uptake mechanism acquires energy for active transport via ATP hydrolysis or via movement against electrochemical gradients. As an oligomeric N-glycan, SERT contains disulfide bonds between cysteine residues on the second extracellular domain. Post-translational modifications regulate the uptake kinetics and membrane trafficking of SERT, in part by regulating the proper folding and assembly of SERT in a host-dependent manner (PMID: 30394319). This antibody detected 70 kDa and 140 kDa bands in SDS-PAGE. The 140 kDa band corresponds to the dimer of high mannose-type SERT (PMID: 19323988).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Front Cell Dev Biol; Impact of Metformin Treatment on Human Placental Energy Production and Oxidative Stress.; Authors - Jane L Tarry-Adkins
mouse	WB	Sci Rep; Sepsis-induced elevation in plasma serotonin facilitates endothelial hyperpermeability.; Authors - Yicong Li ; KO Validated
mouse	WB	J Cell Physiol; Serotonin Transporter Protects the Placental Cells Against Apoptosis in Caspase 3-Independent Pathway.; Authors - Coedy Hadden ; KO Validated
mouse	WB	Heliyon; Comparison of CUMS at different pregnancy stages, maternal separation, and their effects on offspring in postpartum depression mouse models; Authors - Fei Fei
mouse,human	WB,IHC	Cell Rep; SSRI antidepressant citalopram reverses the Warburg effect to inhibit hepatocellular carcinoma by directly targeting GLUT1; Authors - Fangyuan Dong
mouse	IHC	Eur J Neurosci; The alteration of serotonergic markers in the amygdala and raphe nuclei of oestrogen receptor β knock-out female mice; Authors - Daniel Kalinowski