SLITRK1 Polyclonal antibody

SLITRK1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21119-1-AP

Synonyms

KIAA0918, KIAA1910, LRRC12, RP11 395N17.1, SLIT and NTRK like protein 1, SLITRK1

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Validation Data Gallery

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  WB analysis using 21119-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21119-1-AP (SLITRK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 21119-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21119-1-AP (SLITRK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 21119-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21119-1-AP (SLITRK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 21119-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SLITRK1 antibody (21119-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 21119-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using SLITRK1 antibody (21119-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21119-1-AP targets SLITRK1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SLITRK1 fusion protein Ag15461
• Full Name: SLIT and NTRK-like family, member 1
• Calculated Molecular Weight: 696 aa, 78 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC051738
• Gene Symbol: SLITRK1
• Gene ID (NCBI): 114798
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.