SMAD2 Polyclonal antibody

• Featured Product
• KD/KO Validated

SMAD2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

167

Cat no : 12570-1-AP

Synonyms

JV18-1, JV18 1, JV18, hSMAD2, hMAD-2

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Validation Data Gallery

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  WB analysis using 12570-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12570-1-AP (SMAD2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12570-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12570-1-AP (SMAD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 12570-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12570-1-AP (SMAD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skeletal muscle using 12570-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12570-1-AP (SMAD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 12570-1-AP

  IP result of anti-SMAD2 (IP:12570-1-AP, 3ug; Detection:12570-1-AP 1:1000) with HepG2 cells lysate 3000ug.

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  IHC staining of human endometrial cancer using 12570-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 12570-1-AP (SMAD2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 12570-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 12570-1-AP (SMAD2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 12570-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 12570-1-AP (SMAD2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 12570-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12570-1-AP (SMAD2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12570-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12570-1-AP (SMAD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 12570-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12570-1-AP (SMAD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 12570-1-AP

  Immunofluorescent analysis of HepG2 cells, using SMAD2 antibody 12570-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HepG2 using 12570-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMAD2 antibody (12570-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, mouse skeletal muscle tissue, rat skeletal muscle tissue, HEK-293 cells, HT-1080 cells, HUVEC cells, C2C12 cells, C6 cells, HepG2 cells, MCF-7 cells, PC-3 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human colon cancer tissue, human stomach cancer tissue, human endometrial cancer tissue, mouse colon tissue, rat colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 6 publications below
• WB: See 161 publications below
• IHC: See 11 publications below
• IF: See 9 publications below
• IP: See 3 publications below
• CoIP: See 3 publications below
• ChIP: See 1 publications below

Product Information

12570-1-AP targets SMAD2 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SMAD2 fusion protein Ag3237
• Full Name: SMAD family member 2
• Calculated Molecular Weight: 467 aa, 52 kDa
• Observed Molecular Weight: 52-70 kDa
• GenBank Accession Number: BC014840
• Gene Symbol: SMAD2
• Gene ID (NCBI): 4087
• RRID: AB_2193037
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMAD2, also named as MADH2 and MADR2, belongs to the dwarfin/SMAD family, contains 1 MH1 (MAD homology 1) domain and 1 MH2 (MAD homology 2) domain. SMAD2 is a receptor-regulated SMAD(R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. This protein may act as a tumor suppressor in colorectal carcinoma. It is phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, It is phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases, and then able to interact with SMURF2, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, it is phosphorylated on Ser-240 by CaMK2. It is phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin. In response to TGF-beta, it is ubiquitinated by NEDD4L, which promotes its degradation. In response to TGF-beta signaling, it is acetylated on Lys-19 by coactivators, which increases transcriptional activity. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human SMAD2. The molecular weight of unphosphorylated forms of Smad2 is 52 kDa and phosphorylated forms of Smad2 is 58 kDa. (PMID: 9006934). The ubiquitination form of Smad2 is ~70 kDa (PMID: 25998442).

Publications

Species	Application	Title
mouse	WB	Biomaterials; A nano-conductive osteogenic hydrogel to locally promote calcium influx for electro-inspired bone defect regeneration; Authors - Congcong Yu
rat	CoIP	Basic Res Cardiol; The miR-182/Myadm axis regulates hypoxia-induced pulmonary hypertension by balancing the BMP- and TGF-β-signalling pathways in an SMC/EC-crosstalk-associated manner; Authors - Yongyi Bai
human	WB	J Exp Clin Cancer Res; ACTN1 promotes HNSCC tumorigenesis and cisplatin resistance by enhancing MYH9-dependent degradation of GSK-3β and integrin β1-mediated phosphorylation of FAK; Authors - Li Cui
human	WB	J Exp Clin Cancer Res; N6-methyladenosine-modified circSLCO1B3 promotes intrahepatic cholangiocarcinoma progression via regulating HOXC8 and PD-L1; Authors - Jing Li
human	WB	Drug Des Devel Ther; Keloid Patient Plasma-Derived Exosomal hsa_circ_0020792 Promotes Normal Skin Fibroblasts Proliferation, Migration, and Fibrogenesis via Modulating miR-193a-5p and Activating TGF-β1/Smad2/3 Signaling; Authors - Huan Hu
mouse	WB	Theranostics; Elevated IgE promotes cardiac fibrosis by suppressing miR-486a-5p.; Authors - Hongmei Zhao

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