SMAD3 Polyclonal antibody

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  WB analysis of Jurkat using 25494-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25494-1-AP (SMAD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 25494-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25494-1-AP (SMAD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 25494-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 25494-1-AP (SMAD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 25494-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 25494-1-AP (SMAD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 25494-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25494-1-AP (SMAD3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 25494-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25494-1-AP (SMAD3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon cancer using 25494-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25494-1-AP (SMAD3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 25494-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25494-1-AP (SMAD3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 25494-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25494-1-AP (SMAD3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver cancer using 25494-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25494-1-AP (SMAD3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 25494-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25494-1-AP (SMAD3 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 25494-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SMAD3 antibody (25494-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

• Featured Product
• KD/KO Validated

SMAD3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 25494-1-AP

Publications(75)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

JV15 2, HsT17436, HSPC193, hSMAD3, hMAD-3

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Tested Applications

• Positive WB detected in: Jurkat cells, A431 cells, A549 cells, HeLa cells, SH-SY5Y cells
• Positive IHC detected in: human colon cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 69 publications below
• IHC: See 9 publications below
• IF: See 3 publications below

Product Information

25494-1-AP targets SMAD3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, canine, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: SMAD family member 3
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: NM_005902
• Gene Symbol: SMAD3
• Gene ID (NCBI): 4088
• RRID: AB_2880102
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P84022
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMAD3, also named as hMAD 3 or Mad3, is a 425 amino acid protein, which contains one MH1 domain and one MH2 domain. SMAD3 localizes in the nucleus and cytoplasm. SMAD3 plays an essential role in development and maintenance of self-tolerance and is a critical mediator of the TGFB signaling pathway. SMAD3 is involved in TGFB dependent regulation of steroidogenesis and in T-cell response to TGFB.

Publications

Species	Application	Title
mouse	IF	Circulation; Proefferocytic Therapy Promotes Transforming Growth Factor-β Signaling and Prevents Aneurysm Formation.; Authors - Yoko Kojima
human	WB	Cell Death Differ; REGγ ablation impedes dedifferentiation of anaplastic thyroid carcinoma and accentuates radio-therapeutic response by regulating the Smad7-TGF-β pathway.; Authors - Chan Jiao
mouse	WB	Cell Mol Life Sci; SGK3 deficiency in macrophages suppresses angiotensin II-induced cardiac remodeling via regulating Ndufa13-mediated mitochondrial oxidative stress; Authors - Jiayu Ren
human	WB	Oncogene; The β-catenin/TCF-4-LINC01278-miR-1258-Smad2/3 axis promotes hepatocellular carcinoma metastasis.; Authors - Wei-Juan Huang ; KD Validated
human	WB,IHC,IF	Stem Cell Reports; Gene Correction Reverses Ciliopathy and Photoreceptor Loss in iPSC-Derived Retinal Organoids from Retinitis Pigmentosa Patients.; Authors - Wen-Li Deng ; KD Validated
rat	WB	Oxid Med Cell Longev; WJ-39, an Aldose Reductase Inhibitor, Ameliorates Renal Lesions in Diabetic Nephropathy by Activating Nrf2 Signaling.; Authors - Xiaoyu Zhou