A431 cells were subjected to SDS PAGE followed by western blot with 25494-1-AP( SMAD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

A549 cells were subjected to SDS PAGE followed by western blot with 25494-1-AP( SMAD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

HeLa cells were subjected to SDS PAGE followed by western blot with 25494-1-AP( SMAD3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

Jurkat cells were subjected to SDS PAGE followed by western blot with 25494-1-AP( SMAD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25494-1-AP( SMAD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 25494-1-AP( SMAD3 Antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 25494-1-AP( SMAD3 Antibody) at dilution of 1:50 (under 40x lens)

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 25494-1-AP( SMAD3 Antibody) at dilution of 1:200 (under 10x lens).

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 25494-1-AP( SMAD3 Antibody) at dilution of 1:200 (under 40x lens).

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 25494-1-AP( SMAD3 Antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 25494-1-AP( SMAD3 Antibody) at dilution of 1:50 (under 40x lens)

Immunofluorescent analysis of HeLa cells using 25494-1-AP(SMAD3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG