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SMAD3 Antibody 16 Publications

Rabbit Polyclonal| Catalog number: 25494-1-AP

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Con: 51 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

SMAD3 Antibody WB Jurkat cells 25494-1-AP

Jurkat cells were subjected to SDS PAGE followed by western blot with 25494-1-AP( SMAD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

SMAD3 Antibody IHC human colon cancer tissue 25494-1-AP

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 25494-1-AP( SMAD3 Antibody) at dilution of 1:50 (under 40x lens)

SMAD3 Antibody IF HeLa cells 25494-1-AP

Immunofluorescent analysis of HeLa cells using 25494-1-AP(SMAD3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

Species specificity:
human, rat, mouse
Positive WB detected in:
Jurkat cells, A431 cells, A549 cells, HeLa cells, SH-SY5Y cells
Positive IHC detected in:
human colon cancer tissue, human liver cancer tissue
Positive IF detected in:
HeLa cells
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:50-1:500
IF : 1:10-1:100

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
SMAD family member 3
Calculated molecular weight:
48 kDa
Observed molecular weight:
48 kDa
GenBank accession number:
NM_005902
Gene ID (NCBI):
4088
Gene symbol
SMAD3
Synonyms
hMAD 3, hSMAD3, HSPC193, HsT17436, JV15 2, MAD homolog 3, Mad3, MADH3, Mothers against DPP homolog 3, SMAD 3, SMAD family member 3, SMAD3

Background

SMAD3, also named as hMAD 3 or Mad3, is a 425 amino acid protein, which contains one MH1 domain and one MH2 domain. SMAD3 localizes in the nucleus and cytoplasm. SMAD3 plays an essential role in development and maintenance of self-tolerance and is a critical mediator of the TGFB signaling pathway. SMAD3 is involved in TGFB dependent regulation of steroidogenesis and in T-cell response to TGFB.


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