SMAD3 Polyclonal antibody

• Featured Product
• KD/KO Validated

SMAD3 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

62

Cat no : 25494-1-AP

Synonyms

hMAD 3, hSMAD3, HSPC193, HsT17436, JV15 2, MAD homolog 3, Mad3, MADH3, Mothers against DPP homolog 3, SMAD 3, SMAD family member 3, SMAD3

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Validation Data Gallery

• 

  WB analysis of Jurkat using 25494-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25494-1-AP (SMAD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 25494-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25494-1-AP (SMAD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 25494-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 25494-1-AP (SMAD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 25494-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 25494-1-AP (SMAD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 25494-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25494-1-AP (SMAD3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 25494-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25494-1-AP (SMAD3 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 25494-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25494-1-AP (SMAD3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 25494-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25494-1-AP (SMAD3 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 25494-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25494-1-AP (SMAD3 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 25494-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 25494-1-AP (SMAD3 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 25494-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25494-1-AP (SMAD3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 25494-1-AP

  Immunofluorescent analysis of HeLa cells using 25494-1-AP (SMAD3 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Tested Applications

• Positive WB detected in: Jurkat cells, A431 cells, A549 cells, HeLa cells, SH-SY5Y cells
• Positive IHC detected in: human colon cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 57 publications below
• IHC: See 7 publications below
• IF: See 3 publications below

Product Information

25494-1-AP targets SMAD3 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Cited Reactivity: human, mouse, rat, Bovine, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: SMAD family member 3
• Calculated molecular weight: 48 kDa
• Observed molecular weight: 48 kDa
• GenBank accession number: NM_005902
• Gene symbol: SMAD3
• Gene ID (NCBI): 4088
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SMAD3, also named as hMAD 3 or Mad3, is a 425 amino acid protein, which contains one MH1 domain and one MH2 domain. SMAD3 localizes in the nucleus and cytoplasm. SMAD3 plays an essential role in development and maintenance of self-tolerance and is a critical mediator of the TGFB signaling pathway. SMAD3 is involved in TGFB dependent regulation of steroidogenesis and in T-cell response to TGFB.

Publications

Species	Application	Title
human	WB	Cancer Manag Res; TINAGL1 promotes hepatocellular carcinogenesis through the activation of TGF-β signaling-medicated VEGF expression.; Authors - Lu Sun
human	WB	EBioMedicine; POH1 contributes to hyperactivation of TGF-β signaling and facilitates hepatocellular carcinoma metastasis through deubiquitinating TGF-β receptors and caveolin-1.; Authors - Boshi Wang
human	WB	Brain Res Bull; miR-93-5p targets Smad7 to regulate the transforming growth factor-β1/Smad3 pathway and mediate fibrosis in drug-resistant prolactinoma.; Authors - Bin Hu
human	IHC	BMB Rep; MicroRNA-152-5p inhibits proliferation and migration and promotes apoptosis by regulating expression of Smad3 in human keloid fibroblasts.; Authors - Qianqian Pang
human	WB	J Cell Mol Med; The role and mechanism of transforming growth factor beta 3 in human myocardial infarction-induced myocardial fibrosis.; Authors - Ke Xue
Bovine	WB	J Exp Zool B Mol Dev Evol; Inhibiting transforming growth factor-β signaling regulates in vitro maintenance and differentiation of bovine bone marrow mesenchymal stem cells.; Authors - Xin-Xin Zhao

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