Tested Applications
| Positive WB detected in | pig brain tissue, fetal human brain tissue, HEK-293 cells, mouse brain tissue, PC-12 cells, rat brain tissue, SH-SY5Y cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | rat brain tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | PC-12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60159-1-Ig targets SNAP25 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat, chicken |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6695 Product name: Recombinant human SNAP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-206 aa of BC010647 Sequence: MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLDRVEEGMNHINQDMKEAEKNLKDLGKCCGLFICPCNKLKSSDAYKKAWGNNQDGVVASQPARVVDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG Predict reactive species |
| Full Name | synaptosomal-associated protein, 25kDa |
| Calculated Molecular Weight | 23 kDa |
| Observed Molecular Weight | 25-30 kDa |
| GenBank Accession Number | BC010647 |
| Gene Symbol | SNAP25 |
| Gene ID (NCBI) | 6616 |
| RRID | AB_10666318 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P60880 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The synaptosomal associated protein of 25 kD (SNAP-25) was first identified as a major synaptic protein by Wilson and colleagues. The protein interacts with syntaxin and synaptobrevin through its N-terminal and C-terminal -helical domains. Its palmitoylation domain is located in the middle of the molecule that contains four cysteine residues. Mutation of the cysteines abolishes palmitoylation and membrane binding. Several elegant studies using synaptosome preparations and permeabilized PC12 cells have suggested that SNAP-25 may act in the late post-docking steps of exocytosis. By limited proteolysis and in vitro binding assay, it is proposed that the two helix domains act independently and contribute equally to form the SNARE complex with syntaxin and synaptobrevin. It seems that a major regulatory element is located in the C-terminus of SNAP-25. Removing a 9 amino acid sequence of SNAP-25 inhibited neurosecretion in chromaffin cells.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SNAP25 antibody 60159-1-Ig | Download protocol |
| IHC protocol for SNAP25 antibody 60159-1-Ig | Download protocol |
| IP protocol for SNAP25 antibody 60159-1-Ig | Download protocol |
| WB protocol for SNAP25 antibody 60159-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
SNAP25 Monoclonal antibody (3E4B7), catalog number 60159-1-Ig, has accumulated 15 citations across journals including Advanced Materials, Journal of Extracellular Vesicles, Journal of Nanobiotechnology, Journal of Clinical Investigation, Cell Reports, Neuropsychopharmacology, Environmental Pollution, CNS Neuroscience & Therapeutics, Journal of Cell Biology, Investigative Ophthalmology & Visual Science, Biochimica et Biophysica Acta, Brain Communications, CNS Neurological Disorders Drug Targets, and Neuroscience. Associated research fields include Alzheimer's disease, exosome secretion, synaptic vesicle fusion, phase separation, neurodegeneration, psychiatric disorders, retinal injury, and inflammatory pain.
| Species | Application | Title |
|---|---|---|
Adv Mater Noninvasive Optogenetics Realized by iPSC-Derived Tentacled Carrier in Alzheimer's Disease Treatment | ||
J Extracell Vesicles Identification of the SNARE complex that mediates the fusion of multivesicular bodies with the plasma membrane in exosome secretion | ||
J Nanobiotechnology Activation of the Wnt/β-catenin signalling pathway enhances exosome production by hucMSCs and improves their capability to promote diabetic wound healing | ||
J Clin Invest APOE-ε4 synergizes with sleep disruption to accelerate Aβ deposition and Aβ-associated tau seeding and spreading | ||
Cell Rep SNAP25 undergoes phase separation to facilitate the assembly of the synaptic vesicle fusion machinery. | ||
Neuropsychopharmacology Autism risk gene KMT5B deficiency in prefrontal cortex induces synaptic dysfunction and social deficits via alterations of DNA repair and gene transcription. |
Reviews
What customers say
One reviewer (Gaurav Chhetri, August 2025) rated this SNAP25 antibody 5/5 stars. They used it at a 1:3000 dilution on mouse and human brain cells for Western blot and cell culture applications. The reviewer praised its high specificity, strong and clean signal with minimal background, good sensitivity due to high titer, and consistent results across different neuronal samples, calling it a dependable antibody they would recommend for SNAP25 detection.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Gaurav (Verified Customer) (08-26-2025) | I found the Proteintech SNAP25 antibody to be highly specific and reliable. The antibody gave a strong and clean signal with minimal background, making it very effective for Western blot experiments. Its high titer ensures good sensitivity, and I was able to obtain consistent results across different neuronal samples. Overall, it is a dependable antibody that I would recommend for SNAP25 detection.
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