Validation Data Gallery
|Positive WB detected in||pig brain tissue, fetal human brain tissue, PC-12 cells, HEK-293 cells, rat brain tissue, mouse brain tissue|
|Positive IP detected in||mouse brain tissue|
|Positive IHC detected in||rat brain tissue, mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||PC-12 cells|
|Western Blot (WB)||WB : 1:5000-1:20000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:4000|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
60159-1-Ig targets SNAP25 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
|Tested Reactivity||human, mouse, rat, pig|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||SNAP25 fusion protein Ag6695|
|Full Name||synaptosomal-associated protein, 25kDa|
|Calculated molecular weight||23 kDa|
|Observed molecular weight||25-30 kDa|
|GenBank accession number||BC010647|
|Gene ID (NCBI)||6616|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
The synaptosomal associated protein of 25 kD (SNAP-25) was first identified as a major synaptic protein by Wilson and colleagues. The protein interacts with syntaxin and synaptobrevin through its N-terminal and C-terminal -helical domains. Its palmitoylation domain is located in the middle of the molecule that contains four cysteine residues. Mutation of the cysteines abolishes palmitoylation and membrane binding. Several elegant studies using synaptosome preparations and permeabilized PC12 cells have suggested that SNAP-25 may act in the late post-docking steps of exocytosis. By limited proteolysis and in vitro binding assay, it is proposed that the two helix domains act independently and contribute equally to form the SNARE complex with syntaxin and synaptobrevin. It seems that a major regulatory element is located in the C-terminus of SNAP-25. Removing a 9 amino acid sequence of SNAP-25 inhibited neurosecretion in chromaffin cells.
|Product Specific Protocols|
|WB protocol for SNAP25 antibody 60159-1-Ig||Download protocol|
|IHC protocol for SNAP25 antibody 60159-1-Ig||Download protocol|
|IF protocol for SNAP25 antibody 60159-1-Ig||Download protocol|
|IP protocol for SNAP25 antibody 60159-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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