SNAP25 Monoclonal antibody

SNAP25 Monoclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

3E4B7

Cat no : 60159-1-Ig

Synonyms

RIC 4, RIC4, SEC9, SNAP, SNAP 25, SNAP25, SUP, Super protein



Tested Applications

Positive WB detected inpig brain tissue, fetal human brain tissue, PC-12 cells, HEK-293 cells, rat brain tissue, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inrat brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-12 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

60159-1-Ig targets SNAP25 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivity mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SNAP25 fusion protein Ag6695
Full Name synaptosomal-associated protein, 25kDa
Calculated molecular weight 23 kDa
Observed molecular weight 25-30 kDa
GenBank accession numberBC010647
Gene symbol SNAP25
Gene ID (NCBI) 6616
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The synaptosomal associated protein of 25 kD (SNAP-25) was first identified as a major synaptic protein by Wilson and colleagues. The protein interacts with syntaxin and synaptobrevin through its N-terminal and C-terminal -helical domains. Its palmitoylation domain is located in the middle of the molecule that contains four cysteine residues. Mutation of the cysteines abolishes palmitoylation and membrane binding. Several elegant studies using synaptosome preparations and permeabilized PC12 cells have suggested that SNAP-25 may act in the late post-docking steps of exocytosis. By limited proteolysis and in vitro binding assay, it is proposed that the two helix domains act independently and contribute equally to form the SNARE complex with syntaxin and synaptobrevin. It seems that a major regulatory element is located in the C-terminus of SNAP-25. Removing a 9 amino acid sequence of SNAP-25 inhibited neurosecretion in chromaffin cells.

Protocols

Product Specific Protocols
WB protocol for SNAP25 antibody 60159-1-IgDownload protocol
IHC protocol for SNAP25 antibody 60159-1-IgDownload protocol
IF protocol for SNAP25 antibody 60159-1-IgDownload protocol
IP protocol for SNAP25 antibody 60159-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

CNS Neurol Disord Drug Targets

PMID: 30318007

Authors - Fengjin Hao
ratWB,IHC

Neuroscience

SNAP25/syntaxin4/VAMP2/Munc18-1 Complexes in Spinal Dorsal Horn Contributed to Inflammatory Pain.

Authors - Xing-Lian Duan
mouseWB

Neuropsychopharmacology

Autism risk gene KMT5B deficiency in prefrontal cortex induces synaptic dysfunction and social deficits via alterations of DNA repair and gene transcription.

Authors - Zi-Jun Wang