CoraLite® Plus 488-conjugated SNAP25 Monoclonal antibody

SNAP25 Monoclonal Antibody for WB, IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3E4B7

Cat no : CL488-60159

Synonyms

bA416N4.2, dJ1068F16.2, FLJ23079, RIC 4, RIC4, SEC9, SNAP, SNAP 25, SNAP25, SUP, Super protein



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IF/ICC detected inPC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60159 targets SNAP25 in WB, IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SNAP25 fusion protein Ag6695
Full Name synaptosomal-associated protein, 25kDa
Calculated Molecular Weight 23 kDa
Observed Molecular Weight25 kDa
GenBank Accession NumberBC010647
Gene Symbol SNAP25
Gene ID (NCBI) 6616
RRIDAB_2919232
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The synaptosomal associated protein of 25 kD (SNAP-25) was first identified as a major synaptic protein by Wilson and colleagues. The protein interacts with syntaxin and synaptobrevin through its N-terminal and C-terminal -helical domains. Its palmitoylation domain is located in the middle of the molecule that contains four cysteine residues. Mutation of the cysteines abolishes palmitoylation and membrane binding. Several elegant studies using synaptosome preparations and permeabilized PC12 cells have suggested that SNAP-25 may act in the late post-docking steps of exocytosis. By limited proteolysis and in vitro binding assay, it is proposed that the two helix domains act independently and contribute equally to form the SNARE complex with syntaxin and synaptobrevin. It seems that a major regulatory element is located in the C-terminus of SNAP-25. Removing a 9 amino acid sequence of SNAP-25 inhibited neurosecretion in chromaffin cells. In addition, it has been shown that inhibition of neurosecretion by botulinum toxin E can be rescued by a SNAP-25 C-terminal peptide, probably by initiating the formation of a fusion competent SNARE complex.

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 SNAP25 antibody CL488-60159Download protocol
IF protocol for CL Plus 488 SNAP25 antibody CL488-60159Download protocol
Standard Protocols
Click here to view our Standard Protocols