CoraLite®594-conjugated SNAP25 Monoclonal antibody

SNAP25 Monoclonal Antibody for IF, WB

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3E4B7

Cat no : CL594-60159

Synonyms

bA416N4.2, dJ1068F16.2, FLJ23079, RIC 4, RIC4, SEC9, SNAP, SNAP 25, SNAP25, SUP, Super protein



Tested Applications

Positive WB detected inmouse brain tissue
Positive IF detected inPC-12 cells
For other applications, we recommend the unconjugated version of this antibody, 60159-1-Ig

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-60159 targets SNAP25 in WB, IF applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SNAP25 fusion protein Ag6695
Full Name synaptosomal-associated protein, 25kDa
Calculated molecular weight 23 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC010647
Gene symbol SNAP25
Gene ID (NCBI) 6616
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm/614 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The synaptosomal associated protein of 25 kD (SNAP-25) was first identified as a major synaptic protein by Wilson and colleagues. The protein interacts with syntaxin and synaptobrevin through its N-terminal and C-terminal -helical domains. Its palmitoylation domain is located in the middle of the molecule that contains four cysteine residues. Mutation of the cysteines abolishes palmitoylation and membrane binding. Several elegant studies using synaptosome preparations and permeabilized PC12 cells have suggested that SNAP-25 may act in the late post-docking steps of exocytosis. By limited proteolysis and in vitro binding assay, it is proposed that the two helix domains act independently and contribute equally to form the SNARE complex with syntaxin and synaptobrevin. It seems that a major regulatory element is located in the C-terminus of SNAP-25. Removing a 9 amino acid sequence of SNAP-25 inhibited neurosecretion in chromaffin cells. In addition, it has been shown that inhibition of neurosecretion by botulinum toxin E can be rescued by a SNAP-25 C-terminal peptide, probably by initiating the formation of a fusion competent SNARE complex.

Protocols

Product Specific Protocols
WB protocol for CL594 SNAP25 antibody CL594-60159Download protocol
IF protocol for CL594 SNAP25 antibody CL594-60159Download protocol
Standard Protocols
Click here to view our Standard Protocols