SNTA1 Polyclonal antibody

SNTA1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13131-1-AP

Synonyms

Alpha 1 syntrophin, SNT1, SNTA1, Syntrophin 1, TACIP1

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Validation Data Gallery

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  WB analysis using 13131-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13131-1-AP (SNTA1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 13131-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13131-1-AP (SNTA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 13131-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13131-1-AP (SNTA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse heart tissue, rat heart tissue
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13131-1-AP targets SNTA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SNTA1 fusion protein Ag3820
• Full Name: syntrophin, alpha 1 (dystrophin-associated protein A1, 59kDa, acidic component)
• Calculated Molecular Weight: 505 aa, 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC026215
• Gene Symbol: SNTA1
• Gene ID (NCBI): 6640
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SNTA1, also known as Syntrophin 1, SNT1, is cytoplasmic peripheral membrane scaffold proteins that are components of the dystrophin-associated protein complex. SNTA1 is a signal-transducing adaptor protein and serves as a scaffold for various signaling molecules. Alpha-1 syntrophin contains a PDZ domain, two Pleckstrin homology domains, and a 'syntrophin unique' domain. SNTA1 is the high expression in skeletal muscle and heart (PMID: 8576247, 8612778,). The PDZ domain of SNTA1 has been reported to bind to the C-terminal domain of murine cardiac voltage-gated sodium channels (SkM2) causing altering ion channel activity leading to Long QT syndrome(PMID 19684871, 19862833).