SOCS3 Monoclonal antibody

SOCS3 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse and More (2)

Applications

WB, IP, IHC, IF

Conjugate

Unconjugated

Publications

8

CloneNo.

1G2B12

Cat no : 66797-1-Ig

Synonyms

ATOD4, CIS 3, CIS3, Cish3, SOCS 3, SOCS3, SSI 3, SSI3, STAT induced STAT inhibitor 3

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Validation Data Gallery

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  WB analysis of Daudi using 66797-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 66797-1-Ig (SOCS3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66797-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66797-1-Ig (SOCS3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Ramos using 66797-1-Ig

  Ramos cells were subjected to SDS PAGE followed by western blot with 66797-1-Ig (SOCS3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66797-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66797-1-Ig (SOCS3 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66797-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66797-1-Ig (SOCS3 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 66797-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using SOCS3 antibody (66797-1-Ig, Clone: 1G2B12 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: Daudi cells, Jurkat cells, Ramos cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:400-1:1600

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IP: See 1 publications below

Product Information

66797-1-Ig targets SOCS3 in WB, IP, IHC, IF applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SOCS3 fusion protein Ag5386
• Full Name: suppressor of cytokine signaling 3
• Calculated molecular weight: 25 kDa
• Observed molecular weight: 25 kDa
• GenBank accession number: BC060858
• Gene symbol: SOCS3
• Gene ID (NCBI): 9021
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Suppressor of cytokine signaling 3 (SOCS3) is a member of SOCS family that these proteins induced to attenuate cytokine signal transduction in response to signals from a diverse range of cytokines and growth factors. SOCS3 is a negative regulatory protein that inhibits signaling induced by IL-6 via preventing JAK mediated activation of STAT3. However SOCS3 can positively regulate the ERK-MAPK pathway, inhibit the NF-kB pathway (PMID:26429311,25035930). In addition, SOCS3 brings a great impact on immune responses by inhibiting signaling stimulus, such as LPS, type I and type II IFNs, and IL-12. SOCS3 is widely expressed in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney (PMID: 22961088). Dysregulation of SOCS3 functions can cause a variety of diseases, including allergy, autoimmune diseases, inflammation and cancer (PMID:26429311).

Publications

Species	Application	Title
rat	WB, IP	Int J Mol Sci; Leptin Modulates the Response of Brown Adipose Tissue to Negative Energy Balance: Implication of the GH/IGF-I Axis.; Authors - Vicente Barrios
mouse,human	WB,IHC	Acta Pharmacol Sin; Epac activation ameliorates tubulointerstitial inflammation in diabetic nephropathy.; Authors - Wen-Xia Yang ; KD Validated
mouse	WB	Int J Mol Sci; Alterations in Leptin Signaling in Amyotrophic Lateral Sclerosis (ALS).; Authors - Agueda Ferrer-Donato
rat	WB	Ann Transl Med; Phosphorylated nuclear factor erythroid 2-related factor 2 promotes the secretion of C-C motif chemokine ligand 2 and the recruitment of M2 macrophages.; Authors - Zhanghao Huang
human	WB	Front Mol Biosci; Identification of the Prognostic Value Among Suppressor Of Cytokine Signaling Family Members in Kidney Renal Clear Cell Carcinoma.; Authors - Changjiu Li
mouse	IHC,WB	Front Mol Biosci; STAT3 and IL-6 Contribute to Corticosteroid Resistance in an OVA and Ozone-induced Asthma Model with Neutrophil Infiltration.; Authors - Yishu Xue