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SOCS3 Monoclonal antibody
SOCS3 Monoclonal Antibody for IF, IHC, WB, ELISA
Cat no : 66797-1-Ig
Validation Data Gallery
|Positive WB detected in||Daudi cells, Jurkat cells, Ramos cells|
|Positive IHC detected in||human breast cancer tissue, mouse heart tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse heart tissue|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunohistochemistry (IHC)||IHC : 1:250-1:1000|
|Immunofluorescence (IF)||IF : 1:400-1:1600|
|Sample-dependent, check data in validation data gallery|
66797-1-Ig targets SOCS3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human, mouse samples.
|Tested Reactivity||Human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||SOCS3 fusion protein Ag5386|
|Full Name||suppressor of cytokine signaling 3|
|Calculated molecular weight||25 kDa|
|Observed molecular weight||25 kDa|
|GenBank accession number||BC060858|
|Gene ID (NCBI)||9021|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Suppressor of cytokine signaling 3 (SOCS3) is a member of SOCS family that these proteins induced to attenuate cytokine signal transduction in response to signals from a diverse range of cytokines and growth factors. SOCS3 is a negative regulatory protein that inhibits signaling induced by IL-6 via preventing JAK mediated activation of STAT3. However SOCS3 can positively regulate the ERK-MAPK pathway, inhibit the NF-kB pathway (PMID:26429311,25035930). In addition, SOCS3 brings a great impact on immune responses by inhibiting signaling stimulus, such as LPS, type I and type II IFNs, and IL-12. SOCS3 is widely expressed in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney (PMID: 22961088). Dysregulation of SOCS3 functions can cause a variety of diseases, including allergy, autoimmune diseases, inflammation and cancer (PMID:26429311).
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Leptin Modulates the Response of Brown Adipose Tissue to Negative Energy Balance: Implication of the GH/IGF-I Axis.
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Epac activation ameliorates tubulointerstitial inflammation in diabetic nephropathy.
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Alterations in Leptin Signaling in Amyotrophic Lateral Sclerosis (ALS).
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Phosphorylated nuclear factor erythroid 2-related factor 2 promotes the secretion of C-C motif chemokine ligand 2 and the recruitment of M2 macrophages.
Investigation of the mechanism of Isobavachalcone in treating rheumatoid arthritis through a combination strategy of network pharmacology and experimental verification.
Front Mol Biosci
Identification of the Prognostic Value Among Suppressor Of Cytokine Signaling Family Members in Kidney Renal Clear Cell Carcinoma.