SOCS3 Monoclonal antibody

SOCS3 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse and More (2)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1G2B12

Cat no : 66797-1-Ig

Synonyms

ATOD4, CIS 3, CIS3, Cish3, SOCS 3, SOCS3, SSI 3, SSI3, STAT induced STAT inhibitor 3



Tested Applications

Positive WB detected inDaudi cells, Jurkat cells, Ramos cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

66797-1-Ig targets SOCS3 in WB, IP, IHC, IF,ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen SOCS3 fusion protein Ag5386
Full Name suppressor of cytokine signaling 3
Calculated molecular weight 25 kDa
Observed molecular weight 25 kDa
GenBank accession numberBC060858
Gene symbol SOCS3
Gene ID (NCBI) 9021
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Suppressor of cytokine signaling 3 (SOCS3) is a member of SOCS family that these proteins induced to attenuate cytokine signal transduction in response to signals from a diverse range of cytokines and growth factors. SOCS3 is a negative regulatory protein that inhibits signaling induced by IL-6 via preventing JAK mediated activation of STAT3. However SOCS3 can positively regulate the ERK-MAPK pathway, inhibit the NF-kB pathway (PMID:26429311,25035930). In addition, SOCS3 brings a great impact on immune responses by inhibiting signaling stimulus, such as LPS, type I and type II IFNs, and IL-12. SOCS3 is widely expressed in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney (PMID: 22961088). Dysregulation of SOCS3 functions can cause a variety of diseases, including allergy, autoimmune diseases, inflammation and cancer (PMID:26429311).

Protocols

Product Specific Protocols
WB protocol for SOCS3 antibody 66797-1-IgDownload protocol
IHC protocol for SOCS3 antibody 66797-1-IgDownload protocol
IF protocol for SOCS3 antibody 66797-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB, IP

Int J Mol Sci

Leptin Modulates the Response of Brown Adipose Tissue to Negative Energy Balance: Implication of the GH/IGF-I Axis.

Authors - Vicente Barrios
mouse,humanWB,IHC

Acta Pharmacol Sin

Epac activation ameliorates tubulointerstitial inflammation in diabetic nephropathy.

Authors - Wen-Xia Yang
  • KD Validated