CoraLite®594-conjugated SOCS3 Monoclonal antibody

SOCS3 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1G2B12

Cat no : CL594-66797

Synonyms

ATOD4, CIS 3, CIS3, Cish3, SOCS 3, SOCS3, SOCS-3, SSI 3, SSI3, STAT induced STAT inhibitor 3

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Validation Data Gallery

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  IF Staining of mouse heart using CL594-66797

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite®594 SOCS3 antibody (CL594-66797, Clone: 1G2B12 ) at dilution of 1:200.

• 

  IF Staining of mouse heart using CL594-66797

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite®594 SOCS3 antibody (CL594-66797, Clone: 1G2B12 ) at dilution of 1:200.

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  FC experiment of Jurkat using CL594-66797

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human SOCS3 (CL594-66797, Clone:1G2B12) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF detected in: mouse heart tissue
• Positive FC detected in: Jurkat cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66797 targets SOCS3 in IF, FC (Intra) applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SOCS3 fusion protein Ag5386
• Full Name: suppressor of cytokine signaling 3
• Calculated Molecular Weight: 25 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC060858
• Gene Symbol: SOCS3
• Gene ID (NCBI): 9021
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Suppressor of cytokine signaling 3 (SOCS3) is a member of SOCS family that these proteins induced to attenuate cytokine signal transduction in response to signals from a diverse range of cytokines and growth factors. SOCS3 is a negative regulatory protein that inhibits signaling induced by IL-6 via preventing JAK mediated activation of STAT3. However SOCS3 can positively regulate the ERK-MAPK pathway, inhibit the NF-kB pathway (PMID:26429311,25035930). In addition, SOCS3 brings a great impact on immune responses by inhibiting signaling stimulus, such as LPS, type I and type II IFNs, and IL-12. SOCS3 is widely expressed in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney (PMID: 22961088). Dysregulation of SOCS3 functions can cause a variety of diseases, including allergy, autoimmune diseases, inflammation and cancer (PMID:26429311).