SPAM1 Polyclonal antibody

• 

  WB analysis of PC-3 using 11208-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11208-1-AP (SPAM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11208-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11208-1-AP (SPAM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 11208-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11208-1-AP (SPAM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

SPAM1 Polyclonal Antibody for WB, ELISA

Cat No. 11208-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA

Synonyms

HYA1, Hyal PH20, HYAL1, HYAL3, HYAL5, Hyaluronoglucosaminidase PH 20, PH 20, PH20, SPAG15, SPAM1, Sperm adhesion molecule 1, Sperm surface protein PH 20

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Tested Applications

• Positive WB detected in: PC-3 cells, human testis tissue, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

11208-1-AP targets SPAM1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1682

  Product name: Recombinant human SPAM1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 212-511 aa of BC026163

  Sequence: LLRPNHLWGYYLFPDCYNHHYKKPGYNGSCFNVEIKRNDDLSWLWNESTALYPSIYLNTQQSPVAATLYVRNRVREAIRVSKIPDAKSPLPVFAYTRIVFTDQVLKFLSQDELVYTFGETVALGASGIVIWGTLSIMRSMKSCLLLDNYMETILNPYIINVTLAAKMCSQVLCQEQGVCIRKNWNSSDYLHLNPDNFAIQLEKGGKFTVRGKPTLEDLEQFSEKFYCSCYSTLSCKEKADVKDTDAVDVCIADGVCIDAFLKPPMETEEPQIFYNASPSTLSA

• Full Name: SPAM1
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 45-47 kDa
• GenBank Accession Number: BC026163
• Gene Symbol: SPAM1
• Gene ID (NCBI): 6677
• RRID: AB_2194936
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P38567
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPAM1(sperm adhesion molecule 1), also named as Hyal PH20, belongs to the glycosyl hydrolase 56 family. SPAM1 is a GPI-anchored enzyme present on both the sperm plasma membrane and acrosomal membrane. It is involved in sperm-egg adhesion.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
bovine	WB	Int J Biol Macromol; TMT-based quantitative N-glycoproteomic analysis reveals glycoprotein protection can improve the quality of frozen bovine sperm.; Authors - Na Wang