SPATC1 Polyclonal antibody

• 

  WB analysis of human testis using 25861-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 25861-1-AP (SPATC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

SPATC1 Polyclonal Antibody for WB, ELISA

Cat No. 25861-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Synonyms

SPATA15, SPATC1, SPERIOLIN, SPRN

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Tested Applications

• Positive WB detected in: human testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 2 publications below

Product Information

25861-1-AP targets SPATC1 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12157

  Product name: Recombinant human SPATC1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-350 aa of BC053547

  Sequence: MALAPLAEMLTSLQPSATPGSLMSPLTGTLSTLLSGPAPTSQSSPLTSFLTSPIAGPLTGTLASSLGLPSTGTLTPSSLVAGPVAMSQSSPLIAPVMGTVAVSLSSPLLSSTATPPGVSQNLLANPMSNLVLPEAPRLRLAEPLRGGPTGPQSPACVVPTATTKVPLSTEPPQSTQDPEPLSMAFAGAPLQTSTPIGAMGTPAPKTAFSFNTSDTQAQPSAAQEQVVPASVPTSPTTSPTVTVLASAPALAPQVATSYTPSSTTHIAQGAPHPPSRMHNSPTQNLPVPHCPPHNAHSPPRTSSSPASVNDSRGPRTTEPSTKSMMEVERKLAHRKTSKFPENPRESKQLA

• Full Name: spermatogenesis and centriole associated 1
• Calculated Molecular Weight: 500 aa, 52 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC053547
• Gene Symbol: SPATC1
• Gene ID (NCBI): 375686
• RRID: AB_2880273
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q76KD6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	IF	Mol Cell Proteomics; Modification of Crocodile Spermatozoa Refutes the Tenet That Post-testicular Sperm Maturation Is Restricted To Mammals; Authors - Brett Nixon
mouse	IF	Mol Cell Proteomics; Proteomic profiling of crocodile spermatozoa refutes the tenet that post-testicular maturation is restricted to mammals.; Authors - Brett Nixon