SPIN2B Polyclonal antibody, PBS Only

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  WB analysis of mouse brain using 10107-2-AP (same clone as 10107-2-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10107-2-AP (SPIN2B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10107-2-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 10107-2-AP (same clone as 10107-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10107-2-AP (SPIN2B antibody) at dilution of 1:50 (under 25x lens). This data was developed using the same antibody clone with 10107-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 10107-2-AP (same clone as 10107-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10107-2-AP (SPIN2B antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10107-2-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 10107-2-AP (same clone as 10107-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10107-2-AP (SPIN2B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10107-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 10107-2-AP (same clone as 10107-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10107-2-AP (SPIN2B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10107-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 10107-2-AP (same clone as 10107-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SPIN2B antibody (10107-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10107-2-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 10107-2-AP (same clone as 10107-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SPIN2B antibody (10107-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10107-2-PBS in a different storage buffer formulation.

SPIN2B Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 10107-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

SPIN-2B, SPIN2_duplicate, SPIN-2, SPIN2, SPIN 2B

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Product Information

10107-2-PBS targets SPIN2B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0157

  Product name: Recombinant human SPIN2B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 35-223 aa of BC000044

  Sequence: KKQRGRPSSQPRRNIVGCRISHGWKEGDEPITQWKGTVLDQVPINPSLYLVKYDGIDCVYGLELHRDERVLSLKILSDRVASSHISDANLANTIIGKAVEHMFEGEHGSKDEWRGMVLAQAPIMKAWFYITYEKDPVLYMYQLLDDYKEGDLRIMPESSESPPTEREPGGVVDGLIGKHVEYTKEDGSK

• Full Name: spindlin family, member 2B
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC000044
• Gene Symbol: SPIN2B
• Gene ID (NCBI): 474343
• RRID: AB_2196120
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BPZ2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.