SQRDL Polyclonal antibody

• Featured Product
• KD/KO Validated

SQRDL Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

25

Cat no : 17256-1-AP

Synonyms

CGI 44, SQOR, SQRDL

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Validation Data Gallery

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  WB analysis using 17256-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17256-1-AP (SQRDL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17256-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17256-1-AP (SQRDL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17256-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17256-1-AP (SQRDL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17256-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17256-1-AP (SQRDL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17256-1-AP

  IP result of anti-SQRDL (IP:17256-1-AP, 4ug; Detection:17256-1-AP 1:500) with HeLa cells lysate 2000ug.

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  IHC staining of human brain using 17256-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17256-1-AP (SQRDL antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 17256-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17256-1-AP (SQRDL antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human colon cancer using 17256-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 17256-1-AP (SQRDL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 17256-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17256-1-AP (SQRDL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 17256-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17256-1-AP (SQRDL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 17256-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 17256-1-AP (SQRDL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 17256-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17256-1-AP (SQRDL antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 17256-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17256-1-AP (SQRDL antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 17256-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17256-1-AP (SQRDL antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 17256-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17256-1-AP (SQRDL antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 17256-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17256-1-AP (SQRDL antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 17256-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17256-1-AP (SQRDL antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, HT-29 cells, HepG2 cells, THP-1 cells, HepG cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human brain tissue, human testis tissue, human colon cancer tissue, human liver cancer tissue, mouse liver tissue, rat liver tissue, human spleen tissue, human lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 21 publications below
• IHC: See 5 publications below

Product Information

17256-1-AP targets SQRDL in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SQRDL fusion protein Ag10762
• Full Name: sulfide quinone reductase-like (yeast)
• Calculated Molecular Weight: 450 aa, 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC016836
• Gene Symbol: SQRDL
• Gene ID (NCBI): 58472
• RRID: AB_2195894
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SQRDL(Sulfide:quinone oxidoreductase, mitochondrial) catalyzes the oxidation of hydrogen sulfide, with the help of a quinone.Although the SQRDL precursor protein displays a molecular weight of 50 kDa,a prominent band at about 46 kDa is detected by western blot. This discrepancy reflects the cleavage of an N-terminal targeting sequence of 4 kDa during the import of the protein into the mitochondria(PMID:22067608).SQRDL is one of the enzymes involved in H2S signaling in a discrete population of neurons, oligodendrocytes, and endothelial cells.

Publications

Species	Application	Title
mouse	WB	Cell; Endogenous Hydrogen Sulfide Production Is Essential for Dietary Restriction Benefits.; Authors - Christopher Hine ; KD Validated
mouse,rat	WB	Nat Commun; Sulfide catabolism ameliorates hypoxic brain injury.; Authors - Eizo Marutani ; KD Validated
mouse	WB,IHC	Redox Biol; Sulfide:quinone oxidoreductase alleviates ferroptosis in acute kidney injury via ameliorating mitochondrial dysfunction of renal tubular epithelial cells; Authors - Fangfang Cai
mouse	WB	EMBO Mol Med; β-RA reduces DMQ/CoQ ratio and rescues the encephalopathic phenotype in Coq9 R239X mice.; Authors - Agustín Hidalgo-Gutiérrez
human	WB	EMBO Mol Med; Coenzyme Q deficiency causes impairment of the sulfide oxidation pathway.; Authors - Marcello Ziosi
mouse	WB	EMBO Mol Med; CoQ deficiency causes disruption of mitochondrial sulfide oxidation, a new pathomechanism associated with this syndrome.; Authors - Marta Luna-Sánchez