SRPK1 Polyclonal antibody

SRPK1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse





Cat no : 14073-1-AP


SFRS protein kinase 1, SFRSK1, SR protein specific kinase 1, SRPK1

Tested Applications

Positive WB detected inJurkat cells, L02 cells
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman breast cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells
Positive FC detected inMCF-7 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

14073-1-AP targets SRPK1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SRPK1 fusion protein Ag5149
Full Name SFRS protein kinase 1
Calculated molecular weight92.4 kDa
Observed molecular weight 95 kDa
GenBank accession numberBC038292
Gene symbol SRPK1
Gene ID (NCBI) 6732
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SRPK1 (serine-arginine protein kinase 1) is a protein kinase that specifically phosphorylates proteins containing serine-arginine-rich domains. Its substrates include a family of SR proteins that are key regulators of mRNA AS (alternative splicing). SRPK1 may be localized both in the cytoplasm and nucleus and translocates between these compartments under various conditions (e.g. cell cycle and stress). It is able to phosphorylate SR proteins both in cytoplasm and nucleus (the significance of which remains incompletely understood) and thus regulate the activity of these proteins by affecting their ability to bind other proteins and/or RNA as well as their intracellular localization.(PMID:22817743). This protein has 2 isoforms produced by alternative splicing.


Product Specific Protocols
WB protocol for SRPK1 antibody 14073-1-APDownload protocol
IHC protocol for SRPK1 antibody 14073-1-APDownload protocol
IF protocol for SRPK1 antibody 14073-1-APDownload protocol
IP protocol for SRPK1 antibody 14073-1-APDownload protocol
FC protocol for SRPK1 antibody 14073-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Metab

DRAK2 aggravates nonalcoholic fatty liver disease progression through SRSF6-associated RNA alternative splicing.

Authors - Yufeng Li

J Cancer

Clinical and functional significance of CHK1-S, an alternatively spliced isoform of the CHK1 gene, in hepatocellular carcinoma.

Authors - Guanghui Hu

Cell Chem Biol

Novel RNA-Affinity Proteogenomics Dissects Tumor Heterogeneity for Revealing Personalized Markers in Precision Prognosis of Cancer.

Authors - Li Wang

BMC Cancer

MiR-659-3p inhibits osteosarcoma progression and metastasis by inhibiting cell proliferation and invasion via targeting SRPK1

Authors - Yubao Gong