- Featured Product
- KD/KO Validated
KRAP/SSFA2 Polyclonal antibody
KRAP/SSFA2 Polyclonal Antibody for IF, IP, WB, ELISA
Cat no : 14157-1-AP
|Positive WB detected in
|mouse lung tissue, mouse testis tissue, rat lung tissue
|Positive IP detected in
|mouse testis tissue
|Positive IF detected in
|mouse pancreas tissue
|Western Blot (WB)
|WB : 1:500-1:1000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IF : 1:200-1:800
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
14157-1-AP targets KRAP/SSFA2 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, rat, mouse samples.
|human, rat, mouse
|Host / Isotype
|Rabbit / IgG
|KRAP/SSFA2 fusion protein Ag5339
|sperm specific antigen 2
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
KRAP (Ki-ras-induced actin-interacting protein), also known as SSFA2, CS1 or SPAG13, is a 1,259 amino acid cytoplasmic protein associated with filamentous-actin (F-actin). KRAP is strongly expressed in pancreas and testis. It is also present in colon cancer cell lines, and the expression is up-regulated by activated K-Ras. KRAP-deficient (KO) mice display decreased adiposity and improved glucose metabolism, implicating KRAP as a potential target for the prevention or treatment of obesity and related diseases. KRAP has also been suggested to be a potential target for cancer. This antibody recognizes endogenous KRAP, which migrates with an apparent molecular mass of 180 kDa in SDS-PAGE. (PMID: 14673706; 21501587; 21873152)
KRAP tethers IP3 receptors to actin and licenses them to evoke cytosolic Ca2+ signals.
J Cell Sci
Vimentin and the K-Ras-induced actin-binding protein control inositol-(1,4,5)-trisphosphate receptor redistribution during MDCK cell differentiation.
Relationship between phosphorylation of sperm-specific antigen and prognosis of lung adenocarcinoma.
Reprod Biol Endocrinol
A loss-of-function variant in SSFA2 causes male infertility with globozoospermia and failed oocyte activation.
Identification of a novel cuproptosis-related gene signature and integrative analyses in patients with lower-grade gliomas