• Featured Product
  • KD/KO Validated

KRAP/SSFA2 Polyclonal antibody

KRAP/SSFA2 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14157-1-AP

Synonyms

Cleavage signal 1 protein, CS 1, CS1, KIAA1927, KRAP, SPAG13, sperm specific antigen 2, SSFA2



Tested Applications

Positive WB detected inmouse lung tissue, rat testis tissue, mouse testis tissue
Positive IP detected inmouse testis tissue
Positive IF detected inmouse pancreas tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

14157-1-AP targets KRAP/SSFA2 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KRAP/SSFA2 fusion protein Ag5339
Full Name sperm specific antigen 2
Calculated molecular weight 138 kDa
Observed molecular weight 180 kDa
GenBank accession numberBC028706
Gene symbol SSFA2
Gene ID (NCBI) 6744
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KRAP (Ki-ras-induced actin-interacting protein), also known as SSFA2, CS1 or SPAG13, is a 1,259 amino acid cytoplasmic protein associated with filamentous-actin (F-actin). KRAP is strongly expressed in pancreas and testis. It is also present in colon cancer cell lines, and the expression is up-regulated by activated K-Ras. KRAP-deficient (KO) mice display decreased adiposity and improved glucose metabolism, implicating KRAP as a potential target for the prevention or treatment of obesity and related diseases. KRAP has also been suggested to be a potential target for cancer. This antibody recognizes endogenous KRAP, which migrates with an apparent molecular mass of 180 kDa in SDS-PAGE. (PMID: 14673706; 21501587; 21873152)

Protocols

Product Specific Protocols
WB protocol for KRAP/SSFA2 antibody 14157-1-APDownload protocol
IF protocol for KRAP/SSFA2 antibody 14157-1-APDownload protocol
IP protocol for KRAP/SSFA2 antibody 14157-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Nat Commun

KRAP tethers IP3 receptors to actin and licenses them to evoke cytosolic Ca2+ signals.

Authors - Nagendra Babu Thillaiappan
  • KD Validated
canineWB, IF

J Cell Sci

Vimentin and the K-Ras-induced actin-binding protein control inositol-(1,4,5)-trisphosphate receptor redistribution during MDCK cell differentiation.

Authors - Dingli Florent F
  • KD Validated
humanWB,IHC

J Proteomics

Relationship between phosphorylation of sperm-specific antigen and prognosis of lung adenocarcinoma.

Authors - Akiko Okayama
humanWB,IF,CoIP

Reprod Biol Endocrinol

A loss-of-function variant in SSFA2 causes male infertility with globozoospermia and failed oocyte activation.

Authors - Gelin Huang
humanWB

Front Immunol

Identification of a novel cuproptosis-related gene signature and integrative analyses in patients with lower-grade gliomas

Authors - Jia-hao Bao