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  • KD/KO Validated

KRAP/SSFA2 Polyclonal antibody

KRAP/SSFA2 Polyclonal Antibody for WB, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14157-1-AP

Synonyms

SSFA2, ITPRID2, CS-1, CS1, CS 1



Tested Applications

Positive WB detected inmouse lung tissue, mouse testis tissue, rat lung tissue
Positive IP detected inmouse testis tissue
Positive IF-P detected inmouse pancreas tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14157-1-AP targets KRAP/SSFA2 in WB, IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KRAP/SSFA2 fusion protein Ag5339
Full Name sperm specific antigen 2
Calculated Molecular Weight 138 kDa
Observed Molecular Weight 180 kDa
GenBank Accession NumberBC028706
Gene Symbol SSFA2
Gene ID (NCBI) 6744
RRIDAB_2195472
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KRAP (Ki-ras-induced actin-interacting protein), also known as SSFA2, CS1 or SPAG13, is a 1,259 amino acid cytoplasmic protein associated with filamentous-actin (F-actin). KRAP is strongly expressed in pancreas and testis. It is also present in colon cancer cell lines, and the expression is up-regulated by activated K-Ras. KRAP-deficient (KO) mice display decreased adiposity and improved glucose metabolism, implicating KRAP as a potential target for the prevention or treatment of obesity and related diseases. KRAP has also been suggested to be a potential target for cancer. This antibody recognizes endogenous KRAP, which migrates with an apparent molecular mass of 180 kDa in SDS-PAGE. (PMID: 14673706; 21501587; 21873152)

Protocols

Product Specific Protocols
WB protocol for KRAP/SSFA2 antibody 14157-1-APDownload protocol
IF protocol for KRAP/SSFA2 antibody 14157-1-APDownload protocol
IP protocol for KRAP/SSFA2 antibody 14157-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Nat Commun

KRAP tethers IP3 receptors to actin and licenses them to evoke cytosolic Ca2+ signals.

Authors - Nagendra Babu Thillaiappan
  • KD Validated
canineWB, IF

J Cell Sci

Vimentin and the K-Ras-induced actin-binding protein control inositol-(1,4,5)-trisphosphate receptor redistribution during MDCK cell differentiation.

Authors - Dingli Florent F
  • KD Validated
humanWB,IHC

J Proteomics

Relationship between phosphorylation of sperm-specific antigen and prognosis of lung adenocarcinoma.

Authors - Akiko Okayama
humanWB,IF,CoIP

Reprod Biol Endocrinol

A loss-of-function variant in SSFA2 causes male infertility with globozoospermia and failed oocyte activation.

Authors - Gelin Huang
humanWB

Front Immunol

Identification of a novel cuproptosis-related gene signature and integrative analyses in patients with lower-grade gliomas

Authors - Jia-hao Bao