Tested Applications
| Positive WB detected in | mouse lung tissue, mouse testis tissue, rat lung tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse pancreas tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14157-1-AP targets KRAP/SSFA2 in WB, IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, canine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5339 Product name: Recombinant human KRAP, SSFA2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 907-1256 aa of BC028706 Sequence: SAIEMQLRRVLHDIRNSLQNLSQYPMMRGPDPAAAPYSTQKSSVLPLYENTFQELQVMRRSLNLFRTQMMDLELAMLRQQTMVYHHMTEEERFEVDQLQGLRNSVRMELQDLELQLEERLLGLEEQLRAVRMPSPFRSSALMGMCGSRSADNLSCPSPLNVMEPVTELMQEQSYLKSELGLGLGEMGFEIPPGESSESVFSQATSESSSVCSGPSHANRRTGVPSTASVGKSKTPLVARKKVFRASVALTPTAPSRTGSVQTPPDLESSEEVDAAEGAPEVVGPKSEVEEGHGKLPSMPAAEEMHKNVEQDELQQVIREVGMDPISCVILELSMICTGGGVICALEDTCC Predict reactive species |
| Full Name | sperm specific antigen 2 |
| Calculated Molecular Weight | 138 kDa |
| Observed Molecular Weight | 180 kDa |
| GenBank Accession Number | BC028706 |
| Gene Symbol | SSFA2 |
| Gene ID (NCBI) | 6744 |
| RRID | AB_2195472 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P28290 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
KRAP (Ki-ras-induced actin-interacting protein), also known as SSFA2, CS1 or SPAG13, is a 1,259 amino acid cytoplasmic protein associated with filamentous-actin (F-actin). KRAP is strongly expressed in pancreas and testis. It is also present in colon cancer cell lines, and the expression is up-regulated by activated K-Ras. KRAP-deficient (KO) mice display decreased adiposity and improved glucose metabolism, implicating KRAP as a potential target for the prevention or treatment of obesity and related diseases. KRAP has also been suggested to be a potential target for cancer. This antibody recognizes endogenous KRAP, which migrates with an apparent molecular mass of 180 kDa in SDS-PAGE. (PMID: 14673706; 21501587; 21873152)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for KRAP/SSFA2 antibody 14157-1-AP | Download protocol |
| IHC protocol for KRAP/SSFA2 antibody 14157-1-AP | Download protocol |
| IP protocol for KRAP/SSFA2 antibody 14157-1-AP | Download protocol |
| WB protocol for KRAP/SSFA2 antibody 14157-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The KRAP/SSFA2 polyclonal antibody (Cat# 14157-1-AP) has 10 citations published in journals including Nature Communications, Current Biology, Frontiers in Immunology, Reproductive Biology and Endocrinology, Journal of Cell Science, Andrology, Journal of Proteomics, and Cell Structure and Function. Research fields span IP3 receptor–mediated calcium signaling, cuproptosis-related glioma signatures, male infertility and globozoospermia, cell differentiation, lung adenocarcinoma prognosis, and pancreatic acinar cell ER compartment organization.
| Species | Application | Title |
|---|---|---|
Nat Commun KRAP tethers IP3 receptors to actin and licenses them to evoke cytosolic Ca2+ signals.
| ||
Curr Biol A sustained calcium response mediated by IP3 receptor anchoring to the desmosome is essential for apoptotic cell elimination
| ||
Front Immunol Identification of a novel cuproptosis-related gene signature and integrative analyses in patients with lower-grade gliomas | ||
Reprod Biol Endocrinol A loss-of-function variant in SSFA2 causes male infertility with globozoospermia and failed oocyte activation. | ||
J Cell Sci CREB regulates the expression of type 1 inositol 1,4,5-trisphosphate receptors | ||
J Cell Sci Vimentin and the K-Ras-induced actin-binding protein control inositol-(1,4,5)-trisphosphate receptor redistribution during MDCK cell differentiation.
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