CoraLite®594-conjugated KRAP/SSFA2 Polyclonal antibody

KRAP/SSFA2 Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-14157

Synonyms

Cleavage signal 1 protein, CS 1, CS1, DKFZp313O1039, DKFZp779G0129, FLJ45996, KIAA1927, KRAP, KRAP/SSFA2, SPAG13, sperm specific antigen 2, SSFA2

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Validation Data Gallery

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  IF Staining of mouse pancreas using CL594-14157

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using CoraLite®594 KRAP/SSFA2 antibody (CL594-14157) at dilution of 1:200.

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  IF Staining of mouse pancreas using CL594-14157

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using CoraLite®594 KRAP/SSFA2 antibody (CL594-14157) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: mouse pancreas tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-14157 targets KRAP/SSFA2 in IF applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: KRAP/SSFA2 fusion protein Ag5339
• Full Name: sperm specific antigen 2
• Calculated Molecular Weight: 138 kDa
• GenBank Accession Number: BC028706
• Gene Symbol: SSFA2
• Gene ID (NCBI): 6744
• RRID: AB_2883405
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KRAP (Ki-ras-induced actin-interacting protein), also known as SSFA2, CS1 or SPAG13, is a 1,259 amino acid cytoplasmic protein associated with filamentous-actin (F-actin). KRAP is strongly expressed in pancreas and testis. It is also present in colon cancer cell lines, and the expression is up-regulated by activated K-Ras. KRAP-deficient (KO) mice display decreased adiposity and improved glucose metabolism, implicating KRAP as a potential target for the prevention or treatment of obesity and related diseases. KRAP has also been suggested to be a potential target for cancer. This antibody recognizes endogenous KRAP, which migrates with an apparent molecular mass of 180 kDa in SDS-PAGE. (PMID: 14673706; 21501587; 21873152). This antibody is CL594(Ex/Em 593 nm/614 nm) conjugated.