SSTR5 Monoclonal antibody

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  WB analysis using 66772-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66772-1-Ig (SSTR5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66772-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66772-1-Ig (SSTR5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse kidney using 66772-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using SSTR5 antibody (66772-1-Ig, Clone: 2B10D8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse kidney using 66772-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using SSTR5 antibody (66772-1-Ig, Clone: 2B10D8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

SSTR5 Monoclonal Antibody for WB, IF-P, ELISA

Cat No. 66772-1-Ig

Clone No.2B10D8

Publications(5)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (3)

Applications

WB, IF-P, CoIP, ELISA

Synonyms

somatostatin receptor 5, Somatostatin receptor type 5, SS 5 R, SS5 R, SS5R, SST5, SSTR5

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Tested Applications

• Positive WB detected in: HSC-T6 cells, HeLa cells, HEK-293 cells, HepG2 cells, NIH/3T3 cells, Neuro-2a cells
• Positive IF-P detected in: mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

66772-1-Ig targets SSTR5 in WB, IF-P, CoIP, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: SSTR5 fusion protein Ag18615
• Full Name: somatostatin receptor 5
• Calculated Molecular Weight: 364 aa, 39 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC146576
• Gene Symbol: SSTR5
• Gene ID (NCBI): 6755
• RRID: AB_2882118
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P35346
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Somatostatin is a widely distributed peptide with a broad range of biological actions. Two biological forms of somatostatin exist: somatostatin-14 and -28. Somatostatin receptors (SSTR1, 2A and B, 3, 4 and 5) belong to the G protein coupled receptor family and have a wide expression pattern in both normal tissues and solid tumors (PMID: 23872332). SSTR5 has greater affinity for somatostatin-28 than somatostatin-14 (PMID: 8373420).

Publications

Species	Application	Title
		EMBO J; Somatostatin triggers local cAMP and Ca2+ signaling in primary cilia to modulate pancreatic β-cell function; Authors - Ceren Incedal Nilsson
rat	WB	Phytomedicine; Differential effect of polysaccharide and nonpolysaccharide components in Sijunzi decoction on spleen deficiency syndrome and their mechanisms.; Authors - Ping Ma
mouse	WB	Endocr J; Differential effects of β-arrestin1 and β-arrestin2 on somatostatin receptors in murine AtT-20 corticotroph tumor cells.; Authors - Kazunori Kageyama
human	IF	Front Endocrinol (Lausanne); Dimerization of GPCRs: Novel insight into the role of FLNA and SSAs regulating SST2 and SST5 homo- and hetero-dimer formation; Authors - Donatella Treppiedi
human	WB,CoIP	Cell Death Discov; Repositioning Lomitapide to block ZDHHC5-dependant palmitoylation on SSTR5 leads to anti-proliferation effect in preclinical pancreatic cancer models; Authors - Yumeng Wang