ST7L Polyclonal antibody

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  WB analysis using 17567-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17567-1-AP (ST7L antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 17567-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 17567-1-AP (ST7L antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 17567-1-AP

  IP result of anti-ST7L (IP:17567-1-AP, 3ug; Detection:17567-1-AP 1:1500) with mouse testis tissue lysate 5000ug.

ST7L Polyclonal Antibody for WB, IP, ELISA

Cat No. 17567-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human

Applications

WB, IHC, IP, CoIP, ELISA

Synonyms

FAM4B, ST7 related protein, ST7L, ST7R, STLR

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Tested Applications

• Positive WB detected in: L02 cells, MCF-7 cells, T-47D cells
• Positive IP detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Product Information

17567-1-AP targets ST7L in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with mouse, human samples.

• Tested Reactivity: mouse, human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11768

  Product name: Recombinant human ST7L protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 234-575 aa of BC065501

  Sequence: DCATAYVLLAEEEATTIVDAERLFKQALKAGETIYRQSQQCQHQSPQHEAQLRRDTNVLVYIKRRLAMCARKLGRIREAVKIMRDLMKEFPPLTMLNIHENLLESLLELQAYPDVQAVLAKYDDISLPKSAAICYTAALLKTRTVSEKFSPETASRRGLSTAEINAVEAIHRAVEFNPHVPKYLLEMKSLILPPEHILKRGDSEAIAYAFFHLQHWKRIEGALNLLQCTWEGTFRMIPYPLEKGHLFYPYPSCTETADRELLPTFHHVSVYPKKELPLFIHFTAGFCSSTAMIAILTHQFPEIMGIFAKAVLGLWCPQPWASSGFEENTQDLKSEDLGLSSG

• Full Name: suppression of tumorigenicity 7 like
• Calculated Molecular Weight: 575 aa, 65 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC065501
• Gene Symbol: ST7L
• Gene ID (NCBI): 54879
• RRID: AB_2194882
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8TDW4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ST7 (suppressor of tumorigenicity 7) gene has been proposed to be a tumor suppressor gene, product of which is a multi-pass membrane protein belonging to the ST7 family. ST7L (suppressor of tumorigenicity 7 protein-like) gene encodes a multi-pass membrane protein also belonging to the ST7 family, 8 isoforms produced by alternative splicing have been described . However, the molecular function of this gene product has not been determined so far.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,CoIP	Cell Death Dis; MicroRNA-23b functions as an oncogene and activates AKT/GSK3β/β-catenin signaling by targeting ST7L in hepatocellular carcinoma.; Authors - Likun Zhuang
human	WB	J Exp Clin Cancer Res; Long noncoding RNA MIR31HG inhibits hepatocellular carcinoma proliferation and metastasis by sponging microRNA-575 to modulate ST7L expression.; Authors - Shaoying Yan
human	WB	Int J Mol Med; miR-378 functions as an onco-miRNA by targeting the ST7L/Wnt/β-catenin pathway in cervical cancer.; Authors - Shengjie Li
	IHC	Life Sci; Landscape of multiple tissues' gene expression pattern associated with severe sepsis: Genetic insights from Mendelian randomization and trans-omics analysis; Authors - Lei Wang