STAG2 Polyclonal antibody, PBS Only

• 

  WB analysis of Jurkat using 19837-1-AP (same clone as 19837-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19837-1-AP (STAG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19837-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 19837-1-AP (same clone as 19837-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 19837-1-AP (STAG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19837-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 19837-1-AP (same clone as 19837-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 19837-1-AP (STAG2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19837-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 19837-1-AP (same clone as 19837-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 19837-1-AP (STAG2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19837-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 19837-1-AP (same clone as 19837-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 19837-1-AP (STAG2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19837-1-PBS in a different storage buffer formulation.

• 

  IP experiment of MCF-7 using 19837-1-AP (same clone as 19837-1-PBS)

  IP result of anti-STAG2 (IP:19837-1-AP, 4ug; Detection:19837-1-AP 1:3000) with MCF-7 cells lysate 2120 ug. This data was developed using the same antibody clone with 19837-1-PBS in a different storage buffer formulation.

• 

  IP experiment of U-251 using 19837-1-AP (same clone as 19837-1-PBS)

  IP result of anti-STAG2 (IP:19837-1-AP, 4ug; Detection:19837-1-AP 1:3000) with U-251 cells lysate 3320 ug. This data was developed using the same antibody clone with 19837-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 19837-1-AP (same clone as 19837-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19837-1-AP (STAG2 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19837-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 19837-1-AP (same clone as 19837-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19837-1-AP (STAG2 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19837-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 19837-1-AP (same clone as 19837-1-PBS)

  Immunofluorescent analysis of 293 cells, using STAG2 antibody 19837-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 19837-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HEK-293 using 19837-1-AP (same clone as 19837-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using STAG2 antibody (19837-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19837-1-PBS in a different storage buffer formulation.

STAG2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 19837-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Stromal antigen 2, SA2, SA 2, Cohesin subunit SA-2, Cohesin subunit SA 2

---

Product Information

19837-1-PBS targets STAG2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STAG2 fusion protein Ag13825
• Full Name: stromal antigen 2
• Calculated Molecular Weight: 1231 aa, 141 kDa
• Observed Molecular Weight: 130-141 kDa
• GenBank Accession Number: BC001765
• Gene Symbol: STAG2
• Gene ID (NCBI): 10735
• RRID: AB_10666847
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8N3U4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

STAG2, also named as SA2, is a component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex may play a role in spindle pole assembly during mitosis. Mutations in STAG2 appear to be a first step in the transformation of a normal cell into a cancer cell.