STAP1 Polyclonal antibody

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  WB analysis using 31732-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31732-1-AP (STAP1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 31732-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using STAP1 antibody (31732-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

STAP1 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 31732-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

STAP-1, Docking protein BRDG1, BRDG1

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Tested Applications

• Positive WB detected in: Raji cells, Ramos cells
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31732-1-AP targets STAP1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag35196

  Product name: Recombinant human STAP1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 139-293 aa of BC014958

  Sequence: HEVLEREKKRRIETEQSTSVEKEKEPTEDYVDVLNPMPACFYTVSRKEATEMLQKNPSLGNMILRPGSDSRNYSITIRQEIDIPRIKHYKVMSVGQNYTIELEKPVTLPNLFSVIDYFVKETRGNLRPFICSTDENTGQEPSMEGRSEKLKKNPH

• Full Name: signal transducing adaptor family member 1
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC014958
• Gene Symbol: STAP1
• Gene ID (NCBI): 26228
• RRID: AB_3670102
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9ULZ2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Signal transducing adaptor protein 1 (STAP1), also known as BRDG1, contains a proline-rich region, a pleckstrin homology (PH) domain, and a region in the carboxy terminal half with similarity to the Src Homology 2 (SH2) domain. STAP1 acts downstream of the Tec tyrosine kinase and participates in a positive feedback loop by increasing the activity of Tec (PMID: 10518561).