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STAT1 Antibody 24 Publications

Rabbit Polyclonal| Catalog number: 10144-2-AP

Featured Product KD/KO validated

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Con: 20 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

STAT1 Antibody WB multi-cells 10144-2-AP

WB result of anti-STAT1 (10144-2-AP) in different cells lysates

STAT1 Antibody WB PC-3 cells 10144-2-AP

PC-3 cells were subjected to SDS PAGE followed by western blot with 10144-2-AP(STAT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

STAT1 Antibody IP PC-3 cells 10144-2-AP

IP Result of anti-STAT1 (IP:10144-2-AP, 4ug; Detection:10144-2-AP 1:500) with PC-3 cells lysate 3200ug.

STAT1 Antibody IHC human breast cancer tissue 10144-2-AP

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 10144-2-AP( STAT1 Antibody) at dilution of 1:50 (under 40x lens)

STAT1 Antibody IF PC-3 cells 10144-2-AP

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed PC-3 cells using 10144-2-AP(STAT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

STAT1 Antibody FC MCF-7 cells 10144-2-AP

1X10^6 MCF-7 cells were stained with 0.2ug STAT1 antibody (10144-2-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human, rat, mouse
Positive WB detected in:
PC-3 cells, multi-cells
Positive IP detected in:
PC-3 cells
Positive IHC detected in:
human breast cancer tissue, human testis tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
PC-3 cells
Positive FC detected in:
MCF-7 cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
STAT1 fusion protein Ag0199
Full name:
signal transducer and activator of transcription 1, 91kDa
Calculated molecular weight:
83 kDa
Observed molecular weight:
84 kDa
GenBank accession number:
BC002704
Gene ID (NCBI):
6772
Gene symbol
STAT1
Synonyms
ISGF 3, STAT1, STAT91

Background

What is the molecular weight of STAT1? Are there any isoforms of STAT1? Is STAT1 post-translationally modified?

The molecular weight of STAT1 is 91 kDa for full-length STAT1α and 84 kDa for STAT1β, which is devoid of the C-terminal activation domain (PMID: 1502203). Phosphorylation of STAT1 regulates STAT1 activity. Phosphorylation of Tyr701 by Janus kinases (JAKs) triggers STAT1 homo- and hetero-dimerization with other STAT family members and also causes nuclear translocation, while phosphorylation of Ser727 is needed for full activation (PMID: 10851062). STAT1β lacks Ser727 and has therefore been considered to be inactive or acting in a dominant negative manner to STAT1α, although it has been further shown to be capable of transcription activation in vivo (PMID: 24710278).

 What is the subcellular localization of STAT1?

STAT1 is present in the cytoplasm and in the nucleus. Translocation of STAT1 from the cytoplasm to the nucleus is required for its regulation of gene expression and is dependent on phosphorylation (see above). However, low levels of unphosphorylated STAT1, often referred to as U-STAT1, can also be found in the nucleus, where U-STAT1 regulates the stability of heterochromatin by binding to chromatin in monomeric or dimeric states (PMID: 18840523).

 What is the tissue expression pattern of STAT1?

STAT1 is ubiquitously expressed.



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