STAT1 Polyclonal antibody

• Featured Product
• KD/KO Validated

STAT1 Polyclonal Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

103

Reviews (3)

Cat no : 10144-2-AP

Synonyms

ISGF 3, STAT1, STAT91

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Validation Data Gallery

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  WB analysis of multi-cells using 10144-2-AP

  WB result of anti-STAT1 (10144-2-AP) in different cells lysates.

• 

  WB analysis using 10144-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10144-2-AP (STAT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10144-2-AP

  WB result of STAT1 antibody (10144-2-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAT1 transfected HEK-293 cells.

• 

  WB analysis using 10144-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10144-2-AP (STAT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10144-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10144-2-AP (STAT1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 10144-2-AP

  IP Result of anti-STAT1 (IP:10144-2-AP, 4ug; Detection:10144-2-AP 1:500) with PC-3 cells lysate 3200ug.

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  IHC staining of human breast cancer using 10144-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10144-2-AP (STAT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 10144-2-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10144-2-AP (STAT1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 10144-2-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10144-2-AP (STAT1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 10144-2-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10144-2-AP (STAT1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 10144-2-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10144-2-AP (STAT1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 10144-2-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10144-2-AP (STAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of PC-3 using 10144-2-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 10144-2-AP (STAT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of MCF-7 using 10144-2-AP

  1X10^6 MCF-7 cells were stained with 0.2ug STAT1 antibody (10144-2-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: A431 cells, HEK-293 cells, multi-cells, HepG2 cells, A549 cells, k-562 cells, PC-3 cells
• Positive IP detected in: PC-3 cells
• Positive IF detected in: PC-3 cells
• Positive FC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 10 publications below
• WB: See 91 publications below
• IHC: See 3 publications below
• IF: See 10 publications below
• IP: See 6 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Product Information

10144-2-AP targets STAT1 in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: STAT1 fusion protein Ag0199
• Full Name: signal transducer and activator of transcription 1, 91kDa
• Calculated molecular weight: 83 kDa
• Observed molecular weight: 84 kDa
• GenBank accession number: BC002704
• Gene symbol: STAT1
• Gene ID (NCBI): 6772
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

What is the molecular weight of STAT1? Are there any isoforms of STAT1? Is STAT1 post-translationally modified?

The molecular weight of STAT1 is 91 kDa for full-length STAT1α and 84 kDa for STAT1β, which is devoid of the C-terminal activation domain (PMID: 1502203). Phosphorylation of STAT1 regulates STAT1 activity. Phosphorylation of Tyr701 by Janus kinases (JAKs) triggers STAT1 homo- and hetero-dimerization with other STAT family members and also causes nuclear translocation, while phosphorylation of Ser727 is needed for full activation (PMID: 10851062). STAT1β lacks Ser727 and has therefore been considered to be inactive or acting in a dominant negative manner to STAT1α, although it has been further shown to be capable of transcription activation in vivo (PMID: 24710278).

 What is the subcellular localization of STAT1?

STAT1 is present in the cytoplasm and in the nucleus. Translocation of STAT1 from the cytoplasm to the nucleus is required for its regulation of gene expression and is dependent on phosphorylation (see above). However, low levels of unphosphorylated STAT1, often referred to as U-STAT1, can also be found in the nucleus, where U-STAT1 regulates the stability of heterochromatin by binding to chromatin in monomeric or dimeric states (PMID: 18840523).

 What is the tissue expression pattern of STAT1?

STAT1 is ubiquitously expressed.

Publications

Species	Application	Title
mouse	IF	Cell; Opioid-induced fragile-like regulatory T cells contribute to withdrawal; Authors - Yongsheng Zhu
human	WB	Sci Transl Med; TRIB3 reduces CD8+ T cell infiltration and induces immune evasion by repressing the STAT1-CXCL10 axis in colorectal cancer.; Authors - Shuang Shang
mouse	WB	Small; Cell-Free Extracts from Human Fat Tissue with a Hyaluronan-Based Hydrogel Attenuate Inflammation in a Spinal Cord Injury Model through M2 Microglia/Microphage Polarization.; Authors - Guang-Yu Xu
mouse	WB,IF	Nat Commun; Osteoblasts secrete Cxcl9 to regulate angiogenesis in bone.; Authors - Bin Huang
human	WB	Redox Biol; Inhibition of STAT3-ferroptosis negative regulatory axis suppresses tumor growth and alleviates chemoresistance in gastric cancer.; Authors - Shumin Ouyang
human	ChIP	Arthritis Rheumatol; Association of the Polymorphism rs13259960 in SLEAR With Predisposition to Systemic Lupus Erythematosus.; Authors - Zhen Fan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Paul (Verified Customer) (11-24-2022)

Clear band at the expected size range

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: A549 LUAD cells

FH

Alejandro (Verified Customer) (08-22-2022)

Works fine for both WB, with clear bands, and IF, which shows a high specific staining

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1/100 for IF and 1/1000 for WB
• Cell Tissue Type: Mouse embrionary fibroblasts

FH

Slavica (Verified Customer) (10-04-2021)

The antibody worked very well!!!

• Applications: Western Blot
• Cell Tissue Type: SHED cell lines

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