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STAT1 Antibody 20 Publications

Rabbit Polyclonal| Catalog number: 10144-2-AP

Featured Product KD/KO validated

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Con: 20 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
Application not tested? Please see our Guarantee


Images See all

STAT1 Antibody WB multi-cells 10144-2-AP

WB result of anti-STAT1 (10144-2-AP) in different cells lysates

STAT1 Antibody WB PC-3 cells 10144-2-AP

PC-3 cells were subjected to SDS PAGE followed by western blot with 10144-2-AP(STAT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

STAT1 Antibody IP PC-3 cells 10144-2-AP

IP Result of anti-STAT1 (IP:10144-2-AP, 4ug; Detection:10144-2-AP 1:500) with PC-3 cells lysate 3200ug.

STAT1 Antibody IHC human breast cancer tissue 10144-2-AP

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 10144-2-AP( STAT1 Antibody) at dilution of 1:50 (under 40x lens)

STAT1 Antibody IF PC-3 cells 10144-2-AP

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed PC-3 cells using 10144-2-AP(STAT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

STAT1 Antibody FC MCF-7 cells 10144-2-AP

1X10^6 MCF-7 cells were stained with 0.2ug STAT1 antibody (10144-2-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human, rat, mouse
Positive WB detected in:
PC-3 cells, multi-cells
Positive IP detected in:
PC-3 cells
Positive IHC detected in:
human breast cancer tissue, human testis tissue
Positive IF detected in:
PC-3 cells
Positive FC detected in:
MCF-7 cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
STAT1 fusion protein Ag0199
Full name:
signal transducer and activator of transcription 1, 91kDa
Calculated molecular weight:
83 kDa
Observed molecular weight:
84 kDa
GenBank accession number:
BC002704
Gene ID (NCBI):
6772
Gene symbol
STAT1
Synonyms
ISGF 3, STAT1, STAT91

Background

STAT1 (signal transducers and activators of transcription 1) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. Various ligands, including interferons- and - , EGF, PDGF and IL-6, can activate STAT1. STAT1 protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human STAT1.


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