|Positive WB detected in||HeLa cells, HeLa|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human cervical cancer tissue, human heart tissue, human breast cancer tissue, human endometrial cancer tissue, human liver tissue, human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:2000-1:20000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 60199-1-Ig is STAT3 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||STAT3 fusion protein Ag0360|
|Full Name||signal transducer and activator of transcription 3 (acute-phase response factor)|
|Calculated molecular weight||770 aa, 88 kDa|
|Observed molecular weight||88 kDa|
|GenBank accession number||BC000627|
|Gene ID (NCBI)||6774|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of STAT3. This antibody is a mouse monoclonal antibody raised against residues near the N terminus of human STAT3. STAT3 exists three isoforms and the molecular weight of each isoform respectively is 83 kDa, 87 kDa and 88 kDa.
|Product Specific Protocols|
|WB protocol for STAT3 antibody 60199-1-Ig||Download protocol|
|IHC protocol for STAT3 antibody 60199-1-Ig||Download protocol|
|IF protocol for STAT3 antibody 60199-1-Ig||Download protocol|
|IP protocol for STAT3 antibody 60199-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
Brain Res Bull
Inhibition of Connexin43 Hemichannels with Gap19 Protect-s Cerebral Ischemia/Reperfusion Injury via the JAK2/STAT3 Pathway in Mice.
Exosome-transmitted p120-catenin suppresses hepatocellular carcinoma progression via STAT3 pathways.
Cell Death Dis
CCL5 derived from tumor-associated macrophages promotes prostate cancer stem cells and metastasis via activating β-catenin/STAT3 signaling.
Mol Med Rep
Effect of modified Xiaochaihu decoction‑containing serum on HepG2.2.15 cells via the JAK2/STAT3 signaling pathway.
Postoperative resveratrol administration improves prognosis of rat orthotopic glioblastomas.
Berberine Maintains the Neutrophil N1 Phenotype to Reverse Cancer Cell Resistance to Doxorubicin.